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KLH conjugated synthetic peptide derived from human CD54
IgG
Lyophilized or Liquid
Store at -20 °C for one year.
单克隆
无
Human,Mouse,Rat,
1年
bs-0608R
999
北京博奥森生物技术有限公司
Rabbit
WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100-500,
1mg/ml
ICAM1/CD54
细胞间粘附分子-1抗体
 Sample: Hela(Human) Cell Lysate at 30 ug Hela KO ICAM1 (Human) Cell Lysate at 30 ug Primary: Anti-ICAM1/CD54 (bs-0608R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD  Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD  Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56/69 kD  Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56/69 kD  Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-ICAM1/CD54 (bs-0608R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110/58 kD Observed band size: 110/58 kD  Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-CD54 (bs-0608R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD  Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56/69 kD  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD54/ICAM-1 Polyclonal Antibody, Unconjugated(bs-0608R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control (blue line): A431 cells(blue). Primary Antibody (green line): Rabbit Anti-ICAM1/PE-CY7 Conjugated antibody (bs-0608R-PE-CY7) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 . Protocol The cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.  Blank control: mouse thymouses(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA(green).  Blank control: HUVEC cells(blue). Primary Antibody:Rabbit Anti-CD54 antibody(bs-0608R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (bs-0608R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. 已发表文献 [IF=7.901]Shim W et al. Catechin solubilization by spontaneous hydrogen bonding with poly(ethylene glycol) for dry eyetherapeutics. J Control Release. 2019 May 20.IHC-P, Mouse [IF=3.55]Xiong, H., et al. "Glycyrrhizin Ameliorates Imiquimod-Induced Psoriasis-like Skin Lesions in BALB/c Mice and Inhibits TNF-α-Induced ICAM-1 Expression via NF-κB/MAPK in HaCaT Cells." Cellular Physiology and Biochemistry 35.4 (2015): 1335-1346.IHC-P, Mouse [IF=3.414]Li X et al. Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge. In loperamide-induced rats. J Ethnopharmacol. 2019 Aug 10;240:111961. IHC-P, Rat [IF=3.23]Chenna Narendra S, Chalise JP, Magnusson M, Uppugunduri S (2015) Local but Not Systemic Administration of Uridine Prevents Development of Antigen-Induced Arthritis. PLoS ONE 10(10): e0141863IHC-P, Mouse [IF=3.213]Tang et al. The renoprotective effects of berberine and its potential impact on the expression of β-arrestins and ICAM-1/VCAM-1 in streptozocin induced-diabetic nephropathy rats. (2015) J.Diabetes. IHC-P/WB, rat [IF=3.21]Osada-Oka, Mayuko, et al. "Macrophage-derived exosomes induce inflammatory factors in endothelial cells under hypertensive conditions." Hypertension Research (2016).WB, Rat [IF=2.784]Zhang et al. Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway. (2018) Int.J.Mol.Med. 42:3344-3354WB, Human [IF=2.59]Park, Sumin, et al. "Protective effect of 7-O-succinyl macrolactin A against intestinal inflammation is mediated through PI3-kinase/Akt/mTOR and NF-κB signaling pathways." European Journal of Pharmacology (2014).WB, Rat [IF=2.02]Chu, Changbin, et al. "Celastrol protects kidney against ischemia–reperfusion-induced injury in rats." Journal of Surgical Research (2013).IHC-P, Rat [IF=1.93]Tang, Li‐Qin, et al. "The renoprotective effects of berberine and its potential impact on the expression of β‐arrestins and ICAM‐1/VCAM‐1 in streptozocin induced‐diabetic nephropathy rats." Journal of Diabetes (2015).other, [IF=1.69]Wang et al. Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin?induced lung inflammation in a murine model of asthma. (2016) Mol.Med.Rep. 13:4289-302WB, Mouse [IF=1.679]Kim et al. Blocking junctional adhesion molecule C promotes the recovery of cisplatin-induced acute kidney injury. (2017) Korean.J.Intern.Me. 32:1053-1061FCM, Mouse [IF=1.66]Zhang, Xiaomei, et al. "Effect of mild hypothermia on breast cancer cells adhesion and migration." Bioscience Trends 6.6 (2012): 313-324.other, [IF=1.28]Ji, Mingli, et al. "Up-regulation of ICAM-1mRNA and IL-1βmRNA in lung tissues of a rat model of COPD." International Journal of Clinical and Experimental Medicine 8.11 (2016): 21956-21963.IHC-P, Rat [IF=1.27]Ji, Mingli, et al. "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits."Experimental and Therapeutic Medicine 12.1 (2016): 51-58.IHC-P, Rabbit [IF=0.52]Utomo, Pamudji, et al. "Decreasing SDF1-CXCR4 Expression after Adipose-Derived Mesenchymal Stem Cells (ASCS) Treatment Combined with Freeze-Dried Amniotic Membrane Wrapping in Rat Sciatic Nerve Injury." International Journal of ChemTech Research.IF(ICC), Rat [IF=0]Wang et al. Preliminary Research of Relationship between Acute Peritonitis and Celiac Primo Vessels. (2013) Evid.Based.Complement.Alternat.Me. 2013:569161IF(IHC-F), Rat [IF=0]Sumarya, Made, et al. "BETEL LEAF EXTRACTS (PIPER BETLE L.) PREVENT ENDOTHELIAL DYSFUNCTION BY REDUCING THE LEVELS OF URIC ACID AND ICAM-1 EXPRESSION OF THE HYPERURICEMIA WISTAR RATS (RATTUS NORVEGICUS) AORTIC ENDOTHELIAL." Indonesia Journal of Biomedical ScienceIHC-P, Rat |
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试剂,技术服务,细胞库 / 细胞培养,抗体,ELISA 试剂盒
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