- 询价
- Cell Signaling Technology
- 9706
- USA
- 2026年05月29日
- W, IF-F, IF-IC, F
- H,M,R
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- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-Histone H3 (Ser10) (6G3) Mouse mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3
- 应用范围：
W, IF-F, IF-IC, F
- 保质期：
详见说明书
- 适应物种：
H,M,R
- 库存：
大量
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-F=Immunofluorescence (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-F IF-IC F | H M R | Endogenous | 17 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Histone H3 (Ser10) (6G3) Mouse mAb detects endogenous levels of histone H3 only when phosphorylated at serine 10. The antibody does not cross-react with other phosphorylated histones or acetylated histone H3. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3. Western Blotting
Western blot analysis of whole cell lysates from NIH/3T3 cells, untreated or treated with serum plus calyculin A (to induce phosphorylation of H3), using Phospho-Histone H3 (Ser10) (6G3) Mouse mAb (top), Phospho-Histone H3 (Ser10) Antibody #9701 (middle) or Histone H3 Antibody #9712 (bottom). Flow Cytometry
Flow cytometric analysis of pacilitaxel-treated THP1 cells, using Phospho-Histone H3 (Ser10) (6G3) Mouse mAb versus propidium iodide (DNA content). The red population indicates positive Phospho-Histone H3 cells. Flow Cytometry
Flow cytometric analysis of Phospho-Histone H3 (Ser10) (6G3) Mouse mAb staining of untreated (blue) or serum/calyculin-treated (green) Ramos cells compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Phospho-Histone H3 (Ser10) (6G3) Mouse mAb (red) and alpha/beta-Tubulin Antibody #2148 (green) showing different stages of the cell cycle. Nonmitotic (A), prophase (B), metaphase (C) and anaphase (D). IF-F
Confocal immunofluorescent analysis of proliferating/mitotic cells labeled with Phospho-Histone H3 (Ser10) (6G3) Mouse mAb (blue) in the subventricular zone following 4 h reperfusion after cerebral ischemia. Red = EGR1 antibody #4152. Green = Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854. |
| Background | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
Growth factor stimulation results in phosphorylation of histone H3 at ser 10 and this correlated with expression of immediate early genes suggesting that this phosphorylation is associated with transcriptional activation. Although Western
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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