- 询价
- Cell Signaling Technology
- 5259
- USA
- 2025年10月08日
- W, IP, IF-IC, F
- Rabbit
- H,Mk
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- 详细信息
- 技术资料
- 抗体英文名：
PCM-1 (Q15) Antibody
- 抗原：
synthetic peptide corresponding to residues near the amino terminus of human PCM-1 protein
- 应用范围：
W, IP, IF-IC, F
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 适应物种：
H,Mk
- 供应商：
CST
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC F | H (Mk) | Endogenous | 228 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | PCM-1 (Q15) Antibody detects endogenous levels of total PCM-1 protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PCM-1 protein. Antibodies were purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from RD, Jurkat, and A-204 cells using PCM-1 (Q15) Antibody. Flow Cytometry
Flow cytometric analysis of A-204 cells, using PCM-1 (Q15) Antibody (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). IF-IC
Confocal immunofluorescent analysis of A204 cells using PCM-1 (Q15) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | The centrosome is composed of a pair of centrioles surrounded by electron-dense pericentriolar material and functions as the microtubule-organizing center responsible for microtubule nucleation and spindle organization during cell cycle progression (1). Percentriolar material 1 (PCM-1) is a large, 228 kDa protein associated with the centrosome in a cell cycle dependent manner (2). PCM-1 localizes to small cytoplasmic granules called centrosomal satellites (3). PCM-1 is required for the assembly of several centrosomal proteins including centrin, pericentrin, ninein, NEK2, and CEP250 (4-8). Chromosomal translocations involving genes encoding PCM-1 and the tyrosine kinases Ret and Jak2 are associated with some cancers, including papillary thyroid carcinoma and myeloid leukemia (9-11).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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