- 询价
- Cell Signaling Technology
- 9875
- USA
- 2025年11月21日
- W
- All
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
O-GlcNAc (CTD110.6) Mouse mAb
- 抗原：
peptide containing serine-O-linked N-acetylglucosamine (O-GlcNAc)
- 应用范围：
W
- 级别：
详见MSDS文件
- 适应物种：
All
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W | All | Endogenous | Mouse IgM |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | O-GlcNAc (CTD110.6) Mouse mAb specifically recognizes endogenous levels of O-GlcNAc on proteins in β-O-glycosidic linkage to both serine and threonine. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a peptide containing serine-O-linked N-acetylglucosamine (O-GlcNAc). Western Blotting
Western blot analysis of extracts from HeLa cells in the absence or presence of PUGNAC, an inhibitor of N-acetyl-β-d-glucosaminidase, using O-GlcNAc (CTD110.6) Mouse mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). Western Blotting
Western blot analysis of extracts from various cell lines using O-GlcNAc (CTD110.6) Mouse mAb. |
| Background | O-GlcNAcylation is a post-translational modification where β-D-N-acetylglucosamine (GlcNAc) is covalently linked to cytoplasmic and nuclear proteins at serine or threonine residues (1,2). This modification is important in many cellular processes including metabolism, cell growth and morphogenesis, apoptosis and transcription (2,3) and has been implicated to play a critical role in cancer (1). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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