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Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated)
recombinant protein specific to the amino terminus of human Oct-4A protein
ChIP
CST
大量
详见MSDS文件
H,M
详见说明书
1
-20°c
100 ul (10 immunoprecipitations)/carrier free & custom formulation / quantity
规格: | 产品价格: | ¥请询价 | |
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规格: | 100 ul (10 immunoprecipitations) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
more info application references datasheet PDF MSDS PDF protocols
Applications Key: ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
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ChIP | H M | Endogenous | 45 | Rabbit IgG |
Protocols |
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Specificity / Sensitivity | Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) detects endogenous levels of total Oct-4A protein. |
Source / Purification | Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Oct-4A protein. Chromatin IPChromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NTERA-2 cells and either 10 μl of Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) #5232, Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) #5677, Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated), or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human Nanog promoter primers, SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. Chromatin IPChromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 mES cells and either 10 μl of Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse XIST Intron 1 Primers #4659, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. Chromatin IPChromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 μl of Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
Background | Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7).
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Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know! |
Companion Products |
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc. For Research Use Only. Not For Use In Diagnostic Procedures. |
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