- 询价
- Cell Signaling Technology
- 9559
- USA
- 2025年08月18日
- W, IP, IHC-P
- H,M,R,Mk
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
PTEN (138G6) Rabbit mAb
- 抗原：
synthetic peptide corresponding to the carboxy-terminal sequence of human PTEN
- 应用范围：
W, IP, IHC-P
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P | H M R Mk | Endogenous | 54 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | PTEN (138G6) Rabbit mAb detects endogenous levels of total PTEN protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human PTEN. Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® PTEN siRNA I (+), using PTEN Antibody #9552 and p42 MAPK (Erk2) Antibody #9108. PTEN Antibody confirms silencing of PTEN expression, while the p42 MAPK (Erk2) Antibody is used to control for loading and specificity of PTEN siRNA. Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® PTEN siRNA II (+), using PTEN (138G6) Rabbit mAb and β-Actin (13E5) Rabbit mAb #4970. PTEN (138G6) Rabbit mAb confirms silencing of PTEN expression, while the β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of PTEN siRNA. Western Blotting
Western blot analysis of extracts from A431, HeLa, 293, COS, PC12, NIH/3T3 and Mouse Brain cells, using PTEN (138G6) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using Phospho-Akt (Ser473) (736E11) Rabbit mAb (IHC Preferred) (#3787) (left) or PTEN (138G6) Rabbit mAb (right). MDA-MB-468 cells lack PTEN. Note the lack of PTEN staining in the Phospho-Akt positive cells. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and prostate carcinoma (right), using PTEN (138G6) Rabbit mAb. Note the stromal cell staining in the PTEN negative lung carcinoma, and the cancer cell staining in the PTEN positive prostate carcinoma. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PTEN (138G6) Rabbit mAb in the presence of control peptide (left) or PTEN Blocking Peptide #1250 (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded cell pellets demonstrating the specificity of PTEN (138G6) Rabbit mAb: DU145, HT-29 and MCF-7 (PTEN positive) and Jurkat, MDA-MB-468 and LNCaP (PTEN negative). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded xenografts using PTEN (138G6) Rabbit mAb. DU145 (left) and A549 (middle) are PTEN positive cell lines, while U-87MG (right) is PTEN negative. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060 (left) or PTEN (138G6) Rabbit mAb (right). Note the presence of P-Akt staining in the PTEN deficient MDA-MB-468 cells. |
| Background | PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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- 内容
- 询问日期
文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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