- 询价
- Cell Signaling Technology
- 5088
- USA
- 2026年01月05日
- W, IP
- Rabbit
- H,Mk
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- 详细信息
- 技术资料
- 抗体英文名：
Apaf-1 (R205) Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Arg205 of human Apaf-1 protein
- 应用范围：
W, IP
- 宿主：
Rabbit
- 保质期：
详见说明书
- 适应物种：
H,Mk
- 库存：
大量
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H (Mk) | Endogenous | 135 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Apaf-1 (R205) Antibody detects endogenous levels of total Apaf-1 protein. |
| Source / Purification | Polyclonal antibodies are prepared by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg205 of human Apaf-1 protein. Antibodies were purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from THP-1, ACHN, and 293 cells using Apaf-1 (R205) Antibody. |
| Background | Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3 (2,3). The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat (4). Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli (5,6). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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