- ￥1700 - 4000
- GeneTex
- 美国
- GTX629630
- 2025年07月15日
- WB, ICC/IF, IHC-P, IP
- Mouse
- Human, Mouse, Rat, Zebrafish, Yeast, Rabbit, Goat, Drosophila, Cat, Dog, Monkey, Xenopus
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Zebrafish, Yeast, Rabbit, Goat, Drosophila, Cat, Dog, Monkey, Xenopus
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX629630
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IP/MS validation was supported by references (PMID:30377426)
- 抗体英文名：
beta Actin antibody [GT5512]
- 抗体名：
beta Actin 抗体 [GT5512]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg 293T whole cell lysate/extract
D. 30 μg A431 whole cell lysate/extract
E. 30 μg HeLa whole cell lysate/extract
F. 30 μg HepG2 whole cell lysate/extract
G. 30 μg H1299 whole cell lysate/extract
H. 30 μg HCT116 whole cell lysate/extract
I. 30 μg MCF-7 whole cell lysate/extract
J. 30 μg NT2D1 whole cell lysate/extract
K. 30 μg PC-3 whole cell lysate/extract
L. 30 μg U87-MG whole cell lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:20000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg rabbit blood lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
beta Actin antibody [GT5512] detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
beta Actin stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: PG-4 cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody? (GTX629630) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.
Various whole cell extracts (15 | 5 | 2 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] (GTX629630) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg 30 hpf zebrafish lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
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beta actin is a type of cell skeleton protein.Its gene is a house keeping gene.In most of cells，beta actin，such as human skeleton，is expressed in a certain level.THat is the reason that people use beta-actin as the criteria of cell numbers
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