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- ￥1700 - 4000
- GeneTex
- 美国
- GTX103322
- 2025年07月15日
- WB, ICC/IF, IHC-P, FACS, IP, ChIP assay
- Rabbit
- Human, Mouse, Rat, Zebrafish, Alligator, Plant, Bird
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Zebrafish, Alligator, Plant, Bird
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX103322
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, FACS, IP, ChIP assay
- 浓度：
0.41 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
NRF2
- 抗体英文名：
NRF2 antibody [N2C2], Internal
- 抗体名：
NRF2 抗体 [N2C2], Internal
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) MDA-MB-231 nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.
NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% PFA at RT for 15 min.
Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.
NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: Neuro2A cells were fixed in 4% PFA at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.
NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.
Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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