- ￥3220 - 20700
- Sino Biological
- 北京
- 10256-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD64 / FCGR1A Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/50.00 µg/100.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥20700.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥3220.0 |
| 规格： | 100.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：Human CD64 / FCGR1A Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain of human CD64A (NP_000557.1) (Met 1-Pro 288) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized CD64-His (Cat: 10256-H08H) at 2 μg/ml (100 μl/well) can bind human IgG1 (Cat: 10702-HNAC), the EC50 of human IgG1 is 0.5-10ng/mL. 2. Scatchard analysis showed the affinity constant (Kd) of CD64 bound to Human IgG1 was 0.059 nM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 16
蛋白分子量：The recombinant human CD64A consists of 284 amino acids and has a predicted molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant human CD64A is approximately 45-60 kDa due to glycosylation.
蛋白NP号：NP_000557.1
蛋白氨基酸序列：Met1-Pro288
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Anderson KW, et al. HDX-MS and MD Simulations Provide Evidence for Stabilization of the IgG1-FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein Bonds.Journal of molecular biology, PubMed ID: 34890647
3, Martinez FJ, et al. PvDBPII elicits multiple antibody-mediated mechanisms that reduce growth in a Plasmodium vivax challenge trial.NPJ vaccines, PubMed ID: 38184681
Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis of higher?order chromatin structures. Cells are treated with formaldehyde to cross?link chromatin interactions, DNA segments bound by protein
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
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