小鼠 CD86蛋白|CD86 protein|CD86(Mouse, C-His)(产品说明)
蛋白名称:CD86蛋白, CD86 protein
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Glu 245) of mouse CD86 (NP_062261.3) precursor was fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 92 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA . Immobilized mouse CD86 at 20 μg/ml (100 μl/well) can bind mouse CD28 with a linear ranger of 6.4-800 ng/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Val 24
蛋白分子量:The recombinant mouse CD86 consists of 233 amino acids after removal of the signal peptide and has a predicted molecular mass of 26.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD86 is approximately 40-55 kDa due to glycosylation.
蛋白NP号:NP_062261.3
蛋白氨基酸序列:Met1-Glu245
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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3, Zhao, Y; et al. PD-L1: CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways. Immunity, Pubmed: 31757674
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