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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse CD86 / B7-2 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/100.00 µg/200.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥16600.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥2310.0 |
| 规格: | 200.00 µg | 产品价格: | ¥3870.0 |
蛋白名称:CD86蛋白, CD86 protein
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Glu 245) of mouse CD86 (NP_062261.3) precursor was fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 92 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA . Immobilized mouse CD86 at 20 μg/ml (100 μl/well) can bind mouse CD28 with a linear ranger of 6.4-800 ng/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Val 24
蛋白分子量:The recombinant mouse CD86 consists of 233 amino acids after removal of the signal peptide and has a predicted molecular mass of 26.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD86 is approximately 40-55 kDa due to glycosylation.
蛋白NP号:NP_062261.3
蛋白氨基酸序列:Met1-Glu245
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验B7 家族 白细胞分化抗原及其功能 B7―1和B7-2 B7―1和B7―2分别称CD80和CD86,属B7家族成员。胞膜外区结构属IgSF成员,各有1个V样区和C2区,两者在氨基酸水平上有25%同源性。CD80胞质区较短,富含精氨酸,有1个RRES序列,为钙调蛋白依赖的磷酸化位点;CD86胞质区含有3个PKC磷酸化位点。 在活化的T细胞、B细胞和单核细胞表达水平明显增加。IL-4和TNF-7分别上调B细胞和单核细胞CD86表达
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
‐affinity tag antibodies for development and determination of protein display, 2624B (Cell Signaling Technology) Hybridization chamber (Corning, product no. 2551
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