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100 µg/1 mg
| 规格: | 100 µg | 产品价格: | ¥2010.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥13070.0 |
重组人 CD4 / LEU3 蛋白 (His标签)(产品说明)
蛋白名称:Human CD4 / LEU3 Protein (His Tag)
蛋白构建:A DNA sequence encoding the human CD4 (NP_000607.1) extracellular domain (Met 1-Trp 390) was fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to CD4-coated plates (0.8 μg/mL, 100 μL/well), approximately >40% will adhere specifically.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Lys 26
蛋白分子量:The recombinant human CD4 consists of 376 amino acids and has a predicted molecular mass of 42.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD4 is approximately 46 kDa due to glycosylation.
蛋白NP号:NP_000607.1
蛋白氨基酸序列:Met1-Trp390
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Benet HC, et al. Conformation of HIV-1 Envelope Governs Rhesus CD4 Usage and Simian-Human Immunodeficiency Virus Replication. mBio. , PubMed ID: 35012342
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