重组人 EphB4 / HTK 蛋白 (Fc标签)(产品说明)
蛋白名称:Human EphB4 / HTK Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Ala 539) of human EphB4 (NP_004435.3) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized human EFNB2 at 2 μg/ml (100 μl/well) can bind human EphB4-Fc with a linear range of 1.56-12.5 ng/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 16
蛋白分子量:The recombinant human EphB4/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 762 amino acids and has a calculated molecular mass of 83.8 kDa. As a result of glycosylation, the rhEphB4/Fc monomer migrates as an approximately 105-115 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号:NP_004435.3
蛋白氨基酸序列:Met1-Ala539
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.