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- Sino Biological
- 12143-H02H
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：ULBP2蛋白, ULBP2 protein
蛋白构建：A DNA sequence encoding the human ULBP2 (Q9BZM5) (Met 1-Ser 217),without the pro peptide, w was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gly 26
蛋白分子量：The recombinant human ULBP2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 433 amino acids and has a predicted molecular mass of 48.7 kDa. As a result of glycosylation, the apparent molecular mass of rhULBP2/Fc monomer is approximately 58 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：Q9BZM5
蛋白氨基酸序列：Met1-Ser217
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验：也可以-80℃/37℃反复冻融3-5次。 2.7 将肿瘤裂解物加入离心管中，3000rpm，离心10min； 2.8 收取上清，0.22mm滤膜过滤除菌，留样检测蛋白含量及细菌、真菌和支原体； 2.9 -80oC保存备用。 3． CIK细胞的培养及鉴定 3.1 步骤1中获得的PBMC 用无血清培养液调整细胞浓度至2 x 106/ml，置于培养瓶内； 3.2 37℃，5%CO2培养箱中孵育2h，以使单核细胞贴壁; 3.3 收集悬浮细胞，用无血清培养液调整细胞浓度至1-2
2F+DwTquooTcizAj6Sh40arj1e+iWJ2ispgsbT801bWi9OaaVDQ0u7N9Rf22Q7je3ypEkT/aXtAx/4gHh20RbetchuWXizve3YY/3L0LGHHqt8Cva+97zf/vEfvyYb4imWXpTxLz8m4rvgjodswc3X26Xnn2o3LbjZPv/Fv5GGuGa33/ZzO2n2HD17NAEWeUXwmQh44uy32U+uutL+69e+6jjedfsddvSxx3m8xNNFQ67wipqQKHyESZ35
activation: C1QR1, IL31RA, INHA, INHBA, TLR1, TLR4, TLR6. Neutrophil activation: APOA2, IL8, PREX1, PRG3. Natural killer cell activation: CD2, IFNB1, IFNK, IL12B, IL2, IL21R, KIR3DL1, ULBP1, ULBP2, ULBP3. Others: AZU1, CX3CL1, ITIH1, TOLLIP
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