- ￥3870 - 8940
- Sino Biological
- 北京
- 18774-H56H-B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human GIPR Protein (His & AVI Tag), Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg/100 µg
| 规格： | 20 µg | 产品价格： | ￥3870.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥8940.0 |
GIPR重组蛋白|Recombinant Human GIPR Protein (His & AVI Tag), Biotinylated（产品说明）
蛋白名称：Human GIPR Protein (His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Human GIPR (NP_000155.1) (Gly26-Gln138) was expressed with a N-terminal polyhistidine tag followed by an AVI tag.The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：His
蛋白分子量：The recombinant Human GIPR consisting of 138 amino acids and has a calculated molecular mass of 16.16 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 23.3 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_000155.1
蛋白氨基酸序列：Gly26-Gln138
蛋白标签：N-His & AVI
蛋白名称：Human GIPR Protein (His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Human GIPR (NP_000155.1) (Gly26-Gln138) was expressed with a N-terminal polyhistidine tag followed by an AVI tag.The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：His
蛋白分子量：The recombinant Human GIPR consisting of 138 amino acids and has a calculated molecular mass of 16.16 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 23.3 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_000155.1
蛋白氨基酸序列：Gly26-Gln138
蛋白标签：N-His & AVI
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文献和实验相关实验
连接就需要应用较强的增强组织通透性的试剂。增强组织通透性常用的方法如应用稀释的酸洗涤、去垢剂（detergent）或称清洗剂Triton X-100、酒精或某些消化酶如胃蛋白酶、胰蛋白酶、胶原蛋白酶和淀粉酶（diastase）等。这种广泛的去蛋白作用无疑可增强组织的通透性和核酸探针的穿透性，提高杂交信号，但同时也会减低RNA的保存最和影响组织结构的形态，因此，在用量及孵育时间上应慎为掌握。 蛋白酶K（Proteinase K）的消化作用在ISHH中是应用于蛋白消化的关键步骤，其浓度及孵育
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Sino Biological 重组人GIPR蛋白 His&AVI标签 生物素化 gipr antibody
￥3870 - 8940
