- 询价
- Thermo scientific -fermentas
- ER1791
- 2025年10月06日
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| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS | |
| 10X Buffer O | 10X Buffer Tango™ | ||||
| ER1791 | 200 u (10 u/µl) | 1.00 ml | 1.00 ml | ER1791 | |
| Recommended buffer for 100% activity | Optimal temperature | Enzyme activity in Fermentas buffers, % | Tango™ buffer for double digestion | |||||
|---|---|---|---|---|---|---|---|---|
| B (blue) 1X |
G (green) 1X |
O (orange) 1X |
R (red) 1X |
Tango™ (yellow) 1X / 2X |
||||
| O | 37°C | NR | 20-50 | 100 | 50-100 | NR | 100 | 2X |
Lambda DNA
2.0%琼脂糖
516 切割位点
50 mM Tris-HCl (pH 7.5, 37°C), 10 mM MgCl2 , 100 mM NaCl, 0.1 mg/ml BSA。
37°C酶切。
10 mM Tris-HCl (pH 7.4, 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA和50% (v/v)甘油。
备注
Dcm: 无重叠 – 不影响。
CpG: 完全重叠 – 阻止酶切。
EcoKI: 无重叠 – 不影响。
EcoBI: 无重叠 – 不影响。
| Methylation type | Sequence | Cleavage effect |
|---|---|---|
| CpG | CCGC |
阻止酶切 |
| bp from the recognition site to fragment end | ||||
|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 |
| 50-100 | ||||
| Lambda | ΦX174 | M13mp18/19 | pBR322 | puc18/19 | pUC57 |
|---|---|---|---|---|---|
| 516 | 36 | 42 | 67 | 34 | 34 |
| pTZ19R/U | pTZ57R | pBluescriptIIKS(-/+) | pBluescriptIISK(-/+) | pACYC177 | pACYC184 |
| 32 | 32 | 36 | 36 | 32 | 56 |
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文献和实验II (SSII) reverse transcriptase (Life Technologies) 2. Method The protocol below may be used for amplifying individual transcripts from RNA recovered from microdissected Cell populations as described in RNA-based
E.coli Total RNA Labeling Protocol for Spotted Microarray
Total 17ul To the RNA /hexamer mix add 17.0 ml of labeling mix and incubate 10min at RT Add 1.5 ml of appropriate CyDye dUTP (1mM stock) followed by 1.5 ml SSII reverse
Inverse PCR For use with Snyder mTn-lacZ/LEU2 based mutagenesis
1 µl • Use either AciI, AluI, HaeIII, HpaII, RsaI or TaqI for Leu transposon libraries • 37 deg C/ >3 hours (or overnight) • 65 deg C/ 20 min III. Ligations (intramolecular, hopefully)
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