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- Thermo scientific -fermentas
- SO115
- 2025年10月18日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
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M13/pUC sequencing primer (-20), 17-mer
5'-d(GTAAAACGACGGCCAGT)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO100 | 0.1 A260 units, 60 µl (3.3 µg) | SO100 |
M13/pUC reverse sequencing primer (-26), 17-mer
5'-d(CAGGAAACAGCTATGAC)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO101 | 0.1 A260 units, 60 µl (3.3 µg) | SO101 |
M13/pUC sequencing primer (-40), 17-mer
5'-d(GTTTTCCCAGTCACGAC)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO113 | 0.1 A260 units, 60 µl (3.3 µg) | SO113 |
M13/pUC sequencing primer (-46), 22-mer
5'-d(GCCAGGGTTTTCCCAGTCACGA)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO114 | 0.1 A260 units, 45 µl (3.3 µg) | SO114 |
M13/pUC reverse sequencing primer (-46), 24-mer
5'-d(GAGCGGATAACAATTTCACACAGG)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO115 | 0.1 A260 units, 42 µl (3.3 µg) | SO115 |
- Applications
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- 克隆载体(例如pUC19, pTZ19R, pTZ57R, M13mp18, pBluescript II)上lacZ 基因内部多克隆位点的外源DNA片段测序(见附录载体说明)。
- PCR方法筛选克隆。
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文献和实验The Universal Primers and the Shotgun DNA Sequencing Method
For studies in molecular biology, DNA purification has been essential, in particular for DNA sequencing, probing, and mutagenesis. The amplification of DNA in Escherichia coli by cloning vehicles derived from M13mp or pUC made expensive
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers
of each DNA sample (100 ng/ul for M13 templates and 200 ng/ul for pUC templates) into the bottom of the 0.2 ml thin-walled reaction tubes (Robbins Scientific). Use the 1 ul sample for A and C reactions, and the 2 ul sample for G and T reactions
ABI SOLiD sequencing is a form of DNA sequencing. DNA sequencing encompasses biochemical methods for determining the order of the nucleotide bases, adenine, guanine, cytosine, 5-methyl cytosine, and thymine, in a DNA oligonucleotide
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