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pJET1.2 forward sequencing primer, 23-mer
5'-d(CGACTCACTATAGGGAGAGCGGC)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO501 | 0.2 A260 , (6.7 µg) | SO501 |
pJET1.2 reverse sequencing primer, 24-mer
5'-d(AAGAACATCGATTTTCCATGGCAG)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO511 | 0.2 A260 , (6.7 µg) | SO511 |
- Applications
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- pJET1.2载体上eco47IR 基因内部。Eco32I位点的外源DNA片段测序。
- PCR方法筛选克隆。
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文献和实验The Universal Primers and the Shotgun DNA Sequencing Method
For studies in molecular biology, DNA purification has been essential, in particular for DNA sequencing, probing, and mutagenesis. The amplification of DNA in Escherichia coli by cloning vehicles derived from M13mp or pUC made expensive
ABI SOLiD sequencing is a form of DNA sequencing. DNA sequencing encompasses biochemical methods for determining the order of the nucleotide bases, adenine, guanine, cytosine, 5-methyl cytosine, and thymine, in a DNA oligonucleotide
Polymerase Chain Reaction Cycle Sequencing with Degenerate Primers
Among the many techniques of cloning new genes, one approach involves degenerate primers (1 –7 ). The approach usually requires three steps: 1. Using degenerate primers to amplify part of the gene
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