- 询价
- Thermo scientific -fermentas
- R0081
- 2025年10月17日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
大量
|
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| R0081 | 50 nmol (50 µl of 1 mM solution) | R0081 |
Biotin-11-dUTP
分子式 : C28 H41 N6 O17 P3 SNa3
分子量 : 927.6 (酸形式 :861.6)
- Applications
-
- PCR、缺口平移、cDNA合成、随机引物标记和引物延伸等应用中酶促非放射性标记DNA。
Biotin-11-dUTP在Taq DNA Polymerase、phi29 DNA Polymerase、Klenow Fragment, exo-、Klenow Fragment、DNAPolymerase I和逆转录酶催化下掺入新合成DNA产物中。
lambdamax = 289 nm, extinction coefficient = 7.1x103 M-1 x cm-1 (pH 7.0)。
- 功能测试：Taq DNA Polymerase催化的PCR和Klenow Fragment, exo-催化的随机引物法DNA标记。
- HPLC检测纯度超过96%。
- 无内切或外切脱氧核糖核酸酶、核糖核酸酶、磷酸酶及切口酶活性的污染。
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- 作者
- 内容
- 询问日期
文献和实验Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP
The selection of an appropriate labeling reagent for a particular experiment, for the most part, depends on the sensitivity and resolution required. For maximum sensitivity in filter hybridizations radioactive labels, such as phosphorous 32
3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection
In this reaction, the enzyme terminal transferase is used to introduce a short tail of fluorescein nucleotides to the 3′-end of an oligonucleotide (1 ) followed by horseradish peroxidase-catalyzed enhanced chemiluminescent detection
Immunoscreening of gt11 Expression Libraries Using an Avidin-Biotin Detection System
Although the avidin-biotin detection system has been used for immunochemical staining of tissues for some time, its use for amplification in immunoassays was not described until 1979 (1 ). The biological basis for the system is the very tight
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