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- SO120
- 2025年10月18日
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- 文献和实验
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SP6 promoter sequencing primer, 18-mer
5'-d(ATTTAGGTGACACTATAG)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO116 | 0.1 A260 units, 56 µl (3.3 µg) | SO116 |
SP6 promoter sequencing primer, 24-mer
5'-d(CATACGATTTAGGTGACACTATAG)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO117 | 0.1 A260 units, 42 µl (3.3 µg) | SO117 |
T7 promoter sequencing primer, 20-mer
5'-d(TAATACGACTCACTATAGGG)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO118 | 0.1 A260 units, 50 µl (3.3 µg) | SO118 |
T3 promoter sequencing primer, 17-mer
5'-d(ATTAACCCTCACTAAAG)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO119 | 0.1 A260 units, 60 µl (3.3 µg) | SO119 |
T3 promoter sequencing primer, 24-mer
5'-d(GCGCGAAATTAACCCTCACTAAAG)-3'
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SO120 | 0.1 A260 units, 60 µl (3.3 µg) | SO120 |
- Applications
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- RNA polymerase启动子在常用的克隆载体(如pTZ19R, pTZ57R, pBluescript II)中对应序列下游的DNA片段测序(见附录载体说明)。
备注
- 引物不适用于某些质粒载体(部分序列缺失，但是启动子功能保留)。
- 引物未磷酸化处理。
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文献和实验The Universal Primers and the Shotgun DNA Sequencing Method
For studies in molecular biology, DNA purification has been essential, in particular for DNA sequencing, probing, and mutagenesis. The amplification of DNA in Escherichia coli by cloning vehicles derived from M13mp or pUC made expensive
ABI SOLiD sequencing is a form of DNA sequencing. DNA sequencing encompasses biochemical methods for determining the order of the nucleotide bases, adenine, guanine, cytosine, 5-methyl cytosine, and thymine, in a DNA oligonucleotide
Polymerase Chain Reaction Cycle Sequencing with Degenerate Primers
Among the many techniques of cloning new genes, one approach involves degenerate primers (1 –7 ). The approach usually requires three steps: 1. Using degenerate primers to amplify part of the gene
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