- 询价
- Thermo scientific -fermentas
- K0661
- 2025年10月16日
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- 技术资料
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| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| K0661 | for 10 react. | K0661 | |
| K0662 | for 30 react. | K0662 |
- Features
-
- 敏感性高 一 斑点杂交实验时，生物素标记探针可检出0.03 pg同源DNA。
- 背景低 一 优化的洗涤和封闭步骤可降低背景杂交信号。
- 经济 一 非放射性检测无需X光片及其它特殊设备。
- 快速、方便 一 即用型组分。
- Applications
-
- Southern杂交、Northern杂交、点/夹缝杂交、菌落或噬斑筛选。
该产品建议与带正电荷的SensiBlot™ Plus Nylon Membrane (#M1001)配套使用，可确保检测的高灵敏度和低背景。
- Streptavidin-AP Conjugate
- BCIP/NBT Solution
- Blocking Reagent
- 10X Blocking/Washing Buffer
- 10X Detection Buffer
- 详细的操作手册
Biotin DecaLabel™ DNA Labeling Kit标记的Lambda DNA/HindIII探针杂交检测SensiBlot™ Plus Nylon Membrane (#M1002)上的同源DNA。使用Biotin Chromogenic Detection Kit (#K0661)检测探针。
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文献和实验Chromogenic Detection of Aminoglycoside Phosphotransferases
Acquired resistance to aminoglycosides is most frequently due to the presence of the so-called aminoglycoside modifying enzymes (AGME) (1 ) able to catalyze one or more of three general reactions: N-acetylation, O-nucleotidylation and O
Chromogenic In Situ Hybridization in Tumor Pathology
to highly repetitive sequences using radioactively labeled probes that were subsequences visualized by autoradiography. The use of radioisotopes has many disadvantages and has been replaced in DNA ISH by nonradioactive detection methods. The most commonly
(Figure 2). Data generated from this assay is currently in submission. Procedure Part I: Detection of Flag-tagged proteins binding 5´ biotin labeled siRNAs in vitro 1.A minimum of ~4.0×106 cells are required/sample and clearly the more cells to begin
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