• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Chromogenic In Situ Hybridization in Tumor Pathology

        互联网

        696
        Ever since the correlation was found between the pathogenesis of diseases and genomic alterations, molecular cytogenetic techniques have found a place in molecular medicine. These techniques are used in tracing gene and genomic abnormalities that are underlying in the development of cancer and genetic diseases. In 1969, Gall and Pardue introduced a technique known as “in situ hybridization” (ISH) to localize nucleic acids in individual cells (1 ). At that time, the capabilities of ISH were limited to highly repetitive sequences using radioactively labeled probes that were subsequences visualized by autoradiography. The use of radioisotopes has many disadvantages and has been replaced in DNA ISH by nonradioactive detection methods. The most commonly used reporter molecules are haptens, such as biotin and digoxigenin, which can be incorporated easily in the probe DNA. The tagged probes are then detected with labeled antibodies against the specific tag or, as in the case of biotin, with a labeled avidin molecule. Since the first report of fluorescent in situ hybridization (FISH) (2 ), the principle of FISH has remained essentially the same, with the exception that biotin and digoxigenin have partly been replaced by directly fluorochrome-conjugated nucleotides, which simplifies the laboratory protocol.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序