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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
美科美
- 英文名:
ECL Chemiluminescence Detection Kit
- 规格:
PK-MB902-500-500
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文献和实验temp for 1 hour. (Can be left at 4℃O/N here)►Wash briefly 2X then 3X10 min each with TBS-T►Mix an equal volume of detection reagent 1 with detection reagent 2 (usually 2mLs total is sufficient).►Drain excess TBS-T from membrane. Lay membrane on a piece
WESTERN BLOTTING USING CHEMILUMINESCENCE
REAGENTS ECL Western blotting kit (Amersham Life Science; cat# RPN2108): contains second antibodies for both mouse and rabbit, substrate and milk blocker (the milk blocker is not normally used when using ruminant samples). Hybond ECL
Hybridization of Horseradish Peroxidase-Labeled Probes and Detection by Enhanced Chemiluminescence
The use of probes directly labeled with horseradish peroxidase (HRP) in conjunction with enhanced chemiluminescence (ECL) allows a flexible approach to hybridizations and detections. This is shown by the diversity of applications
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