- 询价
- Cell Signaling Technology
- 3165
- USA
- 2026年05月28日
- western blot,免疫沉淀（IP）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 兔
- 人,小鼠
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- 文献和实验
- 技术资料
- 级别：
详见MSDS文件
- 宿主：
兔
- 适应物种：
人,小鼠
- 应用范围：
western blot,免疫沉淀（IP）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 供应商：
CST
- 抗原：
/
- 抗原来源：
/
- 保质期：
详见说明书
- 库存：
大量
- 抗体英文名：
Zap-70 (D1C10E) XP Rabbit mAb
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (8 western blots)/100 ul (20 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /40 ul (8 western blots)/100 ul (20 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥询价 | |
|---|---|---|---|
| 规格： | 40 ul (8 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (20 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 40 ul (8 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (20 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Lymphocyte Signaling
Zap-70 (D1C10E) XP® Rabbit mAb #3165
PhosphoSitePlus ® protein, site, and accession data: ZAP70
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-F F | H M | Endogenous | 70 | Rabbit IgG |
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-F=Immunofluorescence (Frozen) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3165:
- Flow , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity
Zap-70 (D1C10E) XP® Rabbit mAb detects endogenous levels of total Zap-70. This antibody does not cross-react with other related proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human Zap-70.
Western Blotting
Western blot analysis of extracts from Jurkat cells using Zap-70 (D1C10E) XP® Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Ramos B cells (red) and Jurkat T cells (blue), using Zap-70 (D1C10E) XP® Rabbit mAb.
IF-F
Confocal immunofluorescent analysis of human tonsil using Zap-70 (D1C10E) XP® Rabbit mAb (red) showing colocalization with T-cells that have been labeled with CD3 Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
- Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
- Iwashima, M. et al. (1994) Science 263, 1136-1139.
- Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
- Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
- Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
- Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
- Wiestner, A. et al. (2003) Blood 101, 4944-4951.
- Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 2701 Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody
- 2704 Phospho-Zap-70 (Tyr493) Antibody
- 2705 Zap-70 (99F2) Rabbit mAb
- 2709 Zap-70 (L1E5) Mouse mAb
- 2707 Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 2717 Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb
- 7071 Phototope® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
肺癌病人的诊断和个性化治疗提供了有效的筛查工具。同样，以ROS1融合蛋白抗体（克隆号D4D6，货号#3287）为基础的IHC检测技术与断裂FISH方法相比，在非小细胞肺癌上IHC检测的准确度达到100%【5】。如此，我们期待检测ROS1融合变异的临床诊断用IHC试剂盒不久将成功推出。如要了解更多有关ALK和ROS1抗体的验证数据，请点击：ALK (D5F3) XP®Rabbit mAb #3633 和 ROS1 (D4D6) Rabbit mAb #3287。 本文
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