Phospho-Met (Tyr1234/1235) (D26) XP™ Rabbit mAb产品图

Phospho-Met (Tyr1234/1235) (D2

6) XP™ Rabbit mAb
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  • 询价
  • Cell Signaling Technology已认证
  • 3077
  • USA
  • 2026年05月28日
  • western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
  • 兔
  • 人,小鼠,大鼠
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 宿主：

      兔

    • 级别：

      详见MSDS文件

    • 适应物种：

      人,小鼠,大鼠

    • 应用范围：

      western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）

    • 抗原来源：

      /

    • 保质期：

      详见说明书

    • 抗体英文名：

      Phospho-Met (Tyr1234/1235) (D26) XP Rabbit mAb

    • 是否单克隆：

      1

    • 库存：

      大量

    • 供应商：

      CST

    • 抗原：

      /

    • 保存条件：

      -20°c

    • 规格：

      40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> /40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a>

    规格：产品价格：￥询价
    规格：40 ul (4 western blots)产品价格：￥请询价
    规格：100 ul (10 western blots)产品价格：￥请询价
    规格：<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> 产品价格：￥请询价
    规格：40 ul (4 western blots)产品价格：￥请询价
    规格：100 ul (10 western blots)产品价格：￥请询价
    规格：<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> 产品价格：￥请询价
    XP Monoclonal Antibody

    Product Pathways - Tyrosine Kinase / Adaptors

    Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W IP IHC-P IHC-F IF-IC F H M R Endogenous 145 Rabbit

    Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
    Reactivity Key:  H=Human  M=Mouse  R=Rat
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Protocols

    Specificity / Sensitivity

    Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb detects endogenous levels of Met only when phosphorylated at Tyr1234/1235. This antibody may cross-react with overexpressed tyrosine phosphorylated Src proteins in Western blot. The use of this antibody for IF and F applications are only recommended for cells over expressing phospho-Met (Y1234/1235).

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1234/1235 of human Met.

    Western Blotting

    Western Blotting

    Western blot analysis of cell extracts from HeLa cells, untreated or stimulated with HGF, using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (upper) and Met (25H2) Mouse mAb #3127 (lower).

    Western Blotting

    Western Blotting

    Western blot analysis of purified active Ron kinase using a Phospho-Ron (Ser1394) Antibody (A), a Phospho-Ron (Tyr1238) Antibody (B), Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (C) and Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (D). This demonstrates that Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb does not cross-react with phospho-Ron by western analysis.

    IHC-P (paraffin)

    IHC-P (paraffin)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb.


    IHC-P (paraffin)

    IHC-P (paraffin)

    Immunohistochemical analysis of paraffin-embedded xenografts from 3T3-Met (left) and 3T3-Ron cells (right) using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb, indicating that this antibody does not cross-react with activated Ron by immunohistochemistry. Image courtesy of Pfizer, Inc.

    IHC-P (paraffin)

    IHC-P (paraffin)

    Immunohistochemical analysis using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb on SignalSlide™ Phospho-Met (1234/1235) IHC Controls #8118 [MKN45 cells, untreated (left) or SU11274-treated (right)].

    IHC-P (paraffin)

    IHC-P (paraffin)

    Immunohistochemical analysis on Src-transfected NIH/3T3 cells, using a Phospho-Src Family (Tyr416) Antibody (left) or Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (right), indicating that the antibody does not cross-react with Src phosphorylated at Tyr416 via immunohistochemistry.


    IHC-P (paraffin)

    IHC-P (paraffin)

    Immunohisochemical analysis of paraffin-embedded HCC827 xenograft using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb.

    IHC-P (paraffin)

    IHC-P (paraffin)

    Immunohistochemical analysis of paraffin-embedded papillary renal cell carcinoma using Phospho-Met (Tyr1234/1225) (D26) XP® Rabbit mAb.

    IHC-F (frozen)

    IHC-F (frozen)

    Immunohistochemical analysis of frozen MKN45 xenograft using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb.


    Flow Cytometry

    Flow Cytometry

    Flow cytometric analysis of MKN-45 cells, untreated (green) or treated with SU11274 (blue).

    IF-IC

    IF-IC

    Confocal immunofluorescence analysis of MKN45 cells, untreated (left) or treated with SU11274 (1 μM, 3 hours; right), using Phospho-MET (Tyr1234/Tyr1235) (D26) XP® Rabbit mAb. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Background

    Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7).

    1. Cooper, C.S. et al. (1984) Nature 311, 29-33.
    2. Bottaro, D.P. et al. (1991) Science 251, 802-4.
    3. Bardelli, A. et al. (1997) Oncogene 15, 3103-11.
    4. Taher, T.E. et al. (2002) J Immunol 169, 3793-800.
    5. Schaeper, U. et al. (2000) J Cell Biol 149, 1419-32.
    6. Eder, J.P. et al. (2009) Clin Cancer Res 15, 2207-14.
    7. Sattler, M. and Salgia, R. (2009) Update Cancer Ther 3, 109-118.

    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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    图标文献和实验
    该产品被引用文献
    [1]Exploring circular MET RNA as a potential biomarker in tumors exhibiting high MET activity
    PMID:37170152J Exp Clin Cancer Res2023-05-12
    [2]Multimodel preclinical platform predicts clinical response of melanoma to immunotherapy
    PMID:32284588Nat Med2020-05
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