和元生物/AAV载体/腺相关病毒包装

AAV/腺相关病毒/病毒包装/AAV载体/腺相关病毒包装

腺相关病毒（Adeno-associated virus，AAV）是一类无法自主复制、无包膜的二十面体微小病毒，其直径约20-26nm，含有4.7kb左右的线状单链DNA作为基因组。AAV感染约3周可达表达高峰

重组腺相关病毒载体（recombinant AAV, rAAV）是在非致病的野生型AAV基础上改造而成的基因载体，研究中采用的rAAV通常为AAV2型基因组与不同的衣壳蛋白结合产生的组合型重组腺相关病毒载体，即rAAV2/x，其中x为不同衣壳蛋白的血清型。

自互补AAV（Self-complementary AAV, scAAV）是在rAAV的基础上将其编码区域设计成双链DNA，感染细胞后互补部分互补形成双链DNA，不需等待第二链DNA合成，因此scAAV感染后3-5天即可到达表达峰值。

和元生物AAV产品优势


纯度高：＞95% 纯度 内毒素低：＜10 EU/mL 内毒素
周期短：10个工作日 病毒包装 空壳率低：＜5% 空壳率

感染效果



和元生物可提供

定制rAAV：10,000+病毒载体库，涵盖过表达/干扰、CRISPR/Cas9、Cre-loxp系统、Tet-on/off系统、特异性表达系统。

现货rAAV：1,500+现货病毒载体库，涵盖光遗传学、化学遗传学、钙/电压指示剂、神经递质探针、Cre/Flp重组酶等工具类AAV，满足您从示踪标记、生理调控到观察验证的全面灵活服务需求。

rAAV载体常用血清型列表


※注：以上AAV基因型均为2型。和元生物新血清型AAV发现系统AAVneO^TM持续发现新型AAV，请关注和元生物公众号。



常见启动子列表

和元生物有60余种特异性启动子，涉及神经系统、心血管、肝脏、肌肉、肺、脂肪、胰腺、眼等研究领域，满足基础科研实验需求。

部分客户文章列表
[01] Nature medicine. (IF=53.440). Zhu L J, Li T Y, Luo C X, et al. CAPON-nNOS coupling can serve as a target for developing new anxiolytics[J]. 2014, 20(9): 1050-1054. [AAV, 焦虑]

[02] Nature medicine. (IF=53.440). Cao X, Li L P, Wang Q, et al. Astrocyte-derived ATP modulates depressive-like behaviors[J]. 2013, 19(6): 773-777. [AAV, 抑郁]

[03] Science. (IF=47.728). Ren S, Wang Y, Yue F, et al. The paraventricular thalamus is a critical thalamic area for wakefulness[J]. 2018, 362(6413): 429-434. [AAV, 觉醒]

[04] Science. (IF=47.728). Mu D, Deng J, Liu K F, et al. A central neural circuit for itch sensation[J]. 2017, 357(6352): 695-699. [AAV, 痒觉]

[05] Science. (IF=47.728). Zhou T, Zhu H, Fan Z, et al. History of winning remodels thalamo-PFC circuit to reinforce social dominance[J]. 2017, 357(6347): 162-168. [AAV, 社会行为]

[06] Science. (IF=47.728). Liu D, Gu X, Zhu J, et al. Medial prefrontal activity during delay period contributes to learning of a working memory task[J]. 2014, 346(6208): 458-463. [AAV, 学习记忆]

[07] Science. (IF=47.728). Li K, Zhou T, Liao L, et al. βCaMKII in lateral habenula mediates core symptoms of depression[J]. 2013, 341(6149): 1016-1020. [AAV, 抑郁]

[08] Cell. (IF=41.582). Zhu H, Wang N, Yao L, et al. Moderate UV exposure enhances learning and memory by promoting a novel glutamate biosynthetic pathway in the brain[J]. 2018, 173(7): 1716-1727. e17. [AAV, 学习记忆]

[09] Cell. (IF=41.582). Bian W J, Miao W Y, He S J, et al. Coordinated spine pruning and maturation mediated by inter-spine competition for cadherin/catenin complexes[J]. 2015, 162(4): 808-822. [AAV, 神经环路]

[10] Cell. (IF=41.582). Wu H, Yu Y, Huang H, et al. Progressive pulmonary fibrosis is caused by elevated mechanical tension on alveolar stem cells[J]. 2020, 180(1): 107-121. e17. [AAV, 呼吸系统]

[11] Circulation. (IF=29.69).Gao P, You M, Li L, et al. Salt-Induced Hepatic Inflammatory Memory Contributes to Cardiovascular Damage Through Epigenetic Modulation of SIRT3[J]. 2022 Feb;145(5):375-391. [AAV, 肝脏]

[12] Cell Metabolism. (IF=27.287). Lu K, Shi TS, Shen SY,et al. Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression[J]. 2022 Mar 1;34(3):441-457.e7. [AAV, 肝骨轴]

[13] Cell Metabolism. (IF=27.287). Ji Y X, Wang Y, Li P L, et al. A kinome screen reveals that Nemo-like kinase is a key suppressor of hepatic gluconeogenesis[J]. 2021, 33(6): 1171-1186. e9. [AAV、ADV, 肝脏]

[14] Cell Metabolism. (IF=27.287). Li Y, Xu J, Lu Y, et al. DRAK2 aggravates nonalcoholic fatty liver disease progression through SRSF6-associated RNA alternative splicing[J]. 2021, 33(10): 2004-2020. e9. [AAV, 肝脏]

[15] Cell Metabolism. (IF=27.287). Xiao Y Z , Yang M , Xiao Y , et al. Reducing Hypothalamic Stem Cell Senescence Protects against Aging-Associated Physiological Decline[J]. 2020, 31(3):534-548.e5. [AAV，衰老]

[16] Cell Research. (IF=25.617). Gu X, Hu X D, Wang D Q, et al. Treatment of autosomal recessive hearing loss via in vivo CRISPR/Cas9-mediated optimized homology-directed repair in mice [J]. 2022, 0:1–4. [AAV, 遗传性耳聋]

[17] Cell Research. (IF=25.617). Liu Z, Le Q, Lv Y, et al. A distinct D1-MSN subpopulation down-regulates dopamine to promote negative emotional state[J]. 2021: 1-18. [AAV，奖赏/厌恶情绪]

[18] Cell Research. (IF=25.617). Xie C, Zhang Y P, Song L, et al. Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome[J]. 2016, 26(10): 1099-1111. [AAV, 心脏]

[19] Cell Research. (IF=25.617). Ma M, Xiong W, Hu F, et al. A novel pathway regulates social hierarchy via lncRNA AtLAS and postsynaptic synapsin IIb[J]. 2020, 30(2): 105-118. [AAV，社会等级]

[20] Nature Neuroscience. (IF=24.884). Zhu X, Tang H D, Dong W Y, et al. Distinct thalamocortical circuits underlie allodynia induced by tissue injury and by depression-like states[J]. 2021, 24(4): 542-553. [AAV, 抑郁/痛觉]

其他相关应用

光遗传 化学遗传 神经环路示踪 钙离子成像 CRISPR/Cas9

Cre-LoxP RNA干扰 基因过表达 microRNA表达 microRNA抑制