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- Sino Biological
- 北京
- 10539-H09B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CHK1 / CHEK1 Protein (GST Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50 µg
蛋白名称：Human CHK1 / CHEK1 Protein (GST Tag)
蛋白构建：A DNA sequence encoding the human CHK1 (AAM78553.1) (Met1-Thr476) was fused with the GST tag at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 85 % as determined by SDS-PAGE
蛋白活性：The specific activity was determined to be > 15 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant human CHK1/GST chimera consists of 700 amino acids and predicts a molecular mass of 80.7kDa. It migrates as an approxiamtely 80 KDa band as predicted in SDS-PAGE under reducing conditions.
蛋白NP号：AAM78553.1
蛋白氨基酸序列：Met1-Thr476
蛋白标签：N-GST
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Jin, T; et al. Discovery and Development of a Potent, Selective, and Orally Bioavailable CHK1 Inhibitor Candidate: 5-((4-((3-Amino-3-methylbutyl)amino)-5-(trifluoromethyl)pyrimidin-2-yl)amino)picolinonitrile. Journal of medicinal chemistry, Pubmed: 34665631
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
REFERENCES Cox, G.N., Pratt D., McDermott M.J. and van der Slice R.W. 1999. Refolding and characterization of recombinant human soluble CTLA-4 expressed in E. coli. Protein Expr. Purif . 17 : 26-32. Frangoni J
photoprotein aequorin by immobilized metal-ion affinity chromatography, Protein Express. Purif. 27 (2003) 384-390. 33. Y. Shi, C. Jiang, Q. Chen, H. Tang, One-step on-column affinity refolding purification and functional analysis of recombinant human VDAC
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
serumfreeconditioned medium (11) ). 3. Methods When expressing recombinant proteins, the choice of vector hasa major impact on purification strategy, yield, and utility of theproduct. Vectors that link a poly-histidine tag to the recombinant protein typically
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