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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human B7-DC / PD-L2 / CD273 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/100.00 µg/200.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥16740.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥2570.0 |
| 规格: | 200.00 µg | 产品价格: | ¥3870.0 |
蛋白名称:Human B7-DC / PD-L2 / CD273 Protein (His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Pro 219) of human PD-L2 (NP_079515.2) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 98 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized human PD-L2 (Cat: 10292-H08H) at 2 μg/ml (100 μl/well) can bind human PD-1 (Cat: 10377-H03H), the EC50 of human PD-1 is 150-800 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 20
蛋白分子量:The recombinant human PD-L2 consists of 211 amino acids and predicts a molecular mass of 24 kDa. As a result of glycosylation, the apparent molecular mass of rh PD-L2 is approximately 40-45 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_079515.2
蛋白氨基酸序列:Met1-Pro219
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Xu X, et al. A Novel Monoclonal Antibody against PD-1 for the Treatment of Viral Oncogene-Induced Tumors or Other Cancer.Cancers, PubMed ID: 39272910
,PD-1/PD-L1这么值钱,大家一定好奇它到底是什么鬼。 PD-1全名是程序性死亡分子1(programmed death-1)是Ishida等[1]通过消减杂交技术于1992年发现的[2],是CD28家族成员,胞质区含有2个酪氨酸残基,1个位于靠近N端的免疫受体酪氨酸抑制基序(ITIM)中,另一个1个位于靠近C端的免疫受体酪氨酸转化基序(ITSM)中[2]。 PD-L1(CD274)是B7家族成员,是PD-1的配体,属于1型跨膜蛋白,全长290个氨基酸,包含1个IgV样
Isolate or Deplete Human CD4+ T Cells - Whole Blood, Buffy Coat, MNC
/buffy coat to remove interfering soluble factors. (1)Dilute the whole blood or buffy coat in Buffer 1 (1 2). (2) Centrifuge at 600 x g for 10 min at 2-8°C. (3) Discard the plasma fraction/upper layer. Resuspend blood to the original
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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