- 询价
- Cell Signaling Technology
- 4292
- USA
- 2026年05月28日
- W, IP
- Rabbit
- H,M,R
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- 文献和实验
- 技术资料
- 抗体英文名：
PI3 Kinase p85 Antibody
- 抗原：
synthetic peptide corresponding to residues of human PI3 Kinase p85
- 应用范围：
W, IP
- 宿主：
Rabbit
- 适应物种：
H,M,R
- 供应商：
CST
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 85 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | PI3 Kinase p85 Antibody detects endogenous levels of PI3 Kinase p85. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human PI3 Kinase p85. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from U937 and 3T3 cells, using PI3 Kinase p85 Antibody. |
| Background | Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验signaling公司的Phospho-PI3K p85 (Tyr458)/p55(Tyr199) 我自己分析没有条带无非是细胞状态不好，或者抗体不好，不知道还有没有可能是没有加磷酸化酶抑制剂，以至于磷酸化的蛋白被去磷酸化了？ 谢谢啦～ yhwangcams 检测内参蛋白如GAPDH,ACTIN,TUBULIN是否成功？ 用PLB裂解细胞得到的蛋白适合做REPORTER，做WESTERN不太合适。建议可以用其他BUFFER裂解蛋白。
Measuring PI3K Lipid Kinase Activity
. In this chapter, we describe methods to measure the PI3K lipid kinase activity in vitro, which is the standard procedure to test the efficacy of inhibitors.
for detailed information. http://en.wikipedia.org/wiki/Phosphoinositide_3-kinase#Classes chuckdouble perhaps, people cant assess wikipedia in mainland. check it out. a pretty new review of PI3K erythrocyte2005
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