Phospho-GSK-3β (Ser9) Antibody

9336:

Western Blotting

Phospho-GSK-3beta (Ser9) Antibody detects endogenous levels of GSK-3β only when phosphorylated at serine 9. The antibody may cross-react weakly with the phosphorylated form of GSK-3α due to high sequence homology.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence of human GSK-3β. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, λ phosphatase treated or untreated, using Phospho-GSK-3bβ (Ser9) Antibody (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower).

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

1. Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141.
3. Cross, D.A. et al. (1995) Nature 378, 785-789.
4. Nusse, R. (1997) Cell 89, 321-323.
5. Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.

• Crowder, R.J. and Freeman, R.S. (2000) Glycogen synthase kinase-3beta activity is critical for neuronal death caused by inhibiting phosphatidylinositol 3-kinase or Akt but not for death caused by nerve growth factor withdrawal. J. Biol. Chem. 275, 34266-34271. Applications: Western Blotting
• Fasshauer, M. et al. (2000) Essential role of insulin receptor substrate-2 in insulin stimulation of Glut4 translocation and glucose uptake in brown adipocytes. J. Biol. Chem. 275, 25494-25501. Applications: Western Blotting
• Ohteki, T. et al. (2000) Negative regulation of T cell proliferation and C278 interleukin 2 production by the serine threonine kinase GSK-3. J. Exp. Med. 192, 991-104. Applications: Western Blotting
• Okano, J. et al. (2000) Akt/protein kinase B isoforms are differentially regulated by epidermal growth factor stimulation. J. Biol. Chem. 275, 30934-30942. Applications: Western Blotting
• Nakamura, N. et al. (2000) Forkhead transcription factors are critical effectors of cell death and cell cycle arrest downstream of PTEN. Mol. Cell. Biol. 20, 8969-8982. Applications: Western Blotting
• Morfini, G. et al. (2002) Glycogen Synthase Kinase 3 phosphorylates kinesin light chains and negatively regulates kinesin-based motility. EMBO 21 (3), 281-293. Applications: IC-IF
• Vitari, A.C. et al. (2004) Biochem J 378, 257-68. Applications: Western Blotting
• Hirai, S. et al. (2006) J Neurosci 26, 11992-2002. Applications: Western Blotting
• Fujita, H. et al. (2005) Biochem Pharmacol 69, 1773-84. Applications: Western Blotting
• Guertin, D.A. et al. (2009) Cancer Cell 15, 148-59. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 9315 GSK-3β (27C10) Rabbit mAb
• 9316 Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb
• 9323 Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb
• 9916 Phospho-Akt Pathway Antibody Sampler Kit
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.