- 询价
- Cell Signaling Technology
- 4272
- USA
- 2025年08月17日
- W, IHC-P
- Rabbit
- H,M,R,Mk,Dm,B
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Cytochrome c Antibody
- 抗原：
synthetic peptide corresponding to amino acids 67-78 of human cytochrome c
- 应用范围：
W, IHC-P
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 供应商：
CST
- 保质期：
详见说明书
- 库存：
大量
- 适应物种：
H,M,R,Mk,Dm,B
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P | H M R Mk Dm (B) | Endogenous | 14 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Cytochrome c Antibody detects endogenous levels of cytochrome c protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids 67-78 of human cytochrome c. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western analysis of cell extracts from human (HeLa and Jurkat), monkey (COS), mouse (L929), rat (C6) and purified bovine cytochrome c, using Cytochrome c Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Cytochrome c Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Cytochrome c Antibody. |
| Background | Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验广义的细胞色素 c是含有血红素 c的细胞色素之总称。通常是指主要存在于高等动植物、酵母、霉菌等线粒体中的细胞色素 c，起着传递电子的作用。为分子量约 1.3万的碱性蛋白质，还原型的吸收带为 550、 520、 415毫微米，氧化型为 407毫微米。α 因此很早便被研究，并获得结晶。不仅一级结构而且立体结构也已清楚（图 1）。其结构基本上是生物界共有的。由细胞色素 c氧化酶〔细胞色素（ a＋ a3 ）复合体〕氧化。在呼吸链中从细胞色素 c开始接受电子，各种还原酶都已清楚
Cytochrome c Stabilization and Immobilization in Aerogels
structurally intact within the gels during the necessary supercritical fluid processing. However, the heme protein cytochrome c (cyt. c ) forms self-organized superstructures around gold (or silver) nanoparticles in buffer that can be encapsulated within silica
Assessing Cytochrome-c Release from Mitochondria
In recent years substantial evidence has accumulated demonstrating a central regulatory role for mitochondria in cell death (1 ). More recently, mechanisms of cell death, through apoptosis, have been elucidated
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