- 询价
- Cell Signaling Technology
- 2122
- USA
- 2025年08月09日
- W
- Rabbit
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Alpha-Fodrin Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Asp1185 of human alpha-fodrin
- 应用范围：
W
- 宿主：
Rabbit
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
H
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 150, 240 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Alpha-Fodrin Antibody detects endogenous levels of full length alpha-fodrin (240 kDa) and its large fragment (150 kDa) resulting from cleavage at aspartic acid 1185. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp1185 of human alpha-fodrin. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or staurosporine-treated, using Cleaved alpha-Fodrin (Asp1185) Antibody #2121 (left) and alpha-Fodrin Antibody (right). |
| Background | Fodrin (also named nonerythroid spectrin) is a universally expressed membrane-associated cytoskeletal protein consisting of alpha- and beta-subunits (1). This protein is important for maintaining normal membrane structure and supporting cell surface protein function (1). Alpha-fodrin is one of the primary targets cleaved by caspases during apoptosis. The full length 240 kDa protein can be cleaved at several sites within its sequence by activated caspases to yield amino-terminal 150 kDa, carboxy-terminal 120 kDa and 35 kDa major products (2-5). Cleavage of alpha-fodrin leads to membrane malfunction and cell shrinkage.
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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