- 询价
- R&D
- 美国/中国
- xy-3461
- 2025年07月14日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Muscle Specific Actin Monoclonal Antibody (SIGNET)详细信息：
-
Description 
Monoclonal Antibody against Muscle Specific Actin (MSA)
(Formerly Signet Catalog Nos: 461-01, 461-16 L1 Predilute, 461-26 L2 Predilute)Intended Use ** In Vitro Diagnostic (IVD) ** Clone HHF35 Form Tissue Culture Supernatant (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Mouse IsoType Muscle Specific Actin Monoclonal Antibody (SIGNET)IgG1 Specificity The SIGNET monoclonal antibody to Muscle Specific Actin, clone HHF35, reacts with a 42 kDa protein specific for actin present in skeletal, cardiac and smooth muscle. Clone HHF35 stains tumors of smooth muscle, (leiomyosarcomas, leimyomas) and skeletal muscle (rhabdomyosarcomas) origin.
This antibody was developed using muscle actin purified from human myocardium.
Concentrated Format:
SIG-3461-1000 [1 mL]
Prediluted Formats:
SIG-3461-16 [6 mL]
SIG-3461-26 [6 mL]
Both formats are ready-to-use with either a Biotin-based (USA Ultra Streptavidin Detection, SIG-32250) or Biotin-free (ACUITYAdvanced Polymer Detection, SIG-32902) detection systems.Uses Muscle Specific Actin Monoclonal Antibody (SIGNET)This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection [Cat No. SIG-32250]
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: Not required
Incubation: 20 minutes at room temperature
Notes Positive tissue (human): Skeletal muscle - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection [Cat No. SIG-32250]
- Muscle Specific Actin Monoclonal Antibody (SIGNET)
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文献和实验by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
Monoclonal Antibody Affinity Chromatography
. These criteria are often best fulfilled for protein targets by antibody ligands. Monoclonal antibodies are the ligands of choice for most purposes, especially when a protein of unknown properties is being characterized. Monoclonal antibodies fulfill
Murine Monoclonal Antibody Development
Major advances in the analysis of biomolecular structure have emerged from the development of highly specific monoclonal antibodies (MAb) by K�hler and Milstein in 1975 (1 ). MAb are produced from hybridoma cell lines that secrete a single
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