Murine Monoclonal Antibody Development
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Major advances in the analysis of biomolecular structure have emerged from the development of highly specific monoclonal antibodies (MAb) by K�hler and Milstein in 1975 (1 ). MAb are produced from hybridoma cell lines that secrete a single species of antibody to a unique antigen. The advantages of MAb as reagents for antibody engineering are:
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They are extremely pure immunological reagents that can be produced by immunization with a heterogenous antigenic preparation;
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| 2. |
They are homogenous with respect to affinity and specificity;
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The specificity of the antibody is directed to a single epitope;
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The desired MAb can be selected for activity, affinity, and subclass by applying appropriate screening methods; and
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| 5. |
Virtually unlimited quantities of MAb can be produced.
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