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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
- α-Smooth Muscle Actin Monoclonal Antibody (SIGNET)详细信息:
-
Description 
Monoclonal Antibody against α-Smooth Muscle Actin (1A4)
(Formerly Signet Catalog Nos: 460-01, 460-16 L1 Predilute, 460-26 L2 Predilute)Intended Use 
** In Vitro Diagnostic (IVD) ** Clone 
1A4 Form 
Ascites (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host 
Mouse IsoType 
α-Smooth Muscle Actin Monoclonal Antibody (SIGNET)IgG2a Specificity 
The Signet monoclonal antibody is specific for the α-smooth muscle actin isoform.
This antibody was generated against α-smooth muscle actin N-terminal decapeptide.
Concentrated Format:
SIG-3460-1000 [1 mL]
Prediluted Formats:
SIG-3460-16 [6 mL] is ready to use with Peroxidase Anti- Peroxidase detection systems such as Signet’s PAP (SIG-32140).
SIG-3460-26 [6 mL] is ready-to-use with Biotin-based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).Uses 
α-Smooth Muscle Actin Monoclonal Antibody (SIGNET)This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution 
The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as Signets USA Ultrastreptavidin Detection.
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: None required
Incubation: 20 minutes at room temperature
Notes 
Positive tissue (human): Smooth muscle Storage 
Store between 2-8°C. Warranty/Conditions 
Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as Signets USA Ultrastreptavidin Detection.
- α-Smooth Muscle Actin Monoclonal Antibody (SIGNET)
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文献和实验by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇:make an Antibody against a Peptides 下一篇:Immunization Protocol for Monoclonal Antibody Production
Monitoring Duchenne Muscular Dystrophy Gene Therapy with Epitope-Specific Monoclonal Antibodies
, though ineffective. The choice of a monoclonal antibody (mAb) to distinguish successful therapy from naturally occurring “revertant” fibres depends on which dystrophin exons are deleted in the DMD patient. Over the past 20 years, we have produced over 150 “exon
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