• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Monoclonal Antibody Affinity Chromatography

        互联网

        2476
        Affinity chromatography is one of the most convenient methods of purifying biological molecules, with many wide ranging applications (1 , 2 ). The main prerequisite is to have a ligand that specifically binds to the target molecule. This ligand must also have two key properties other than exquisite specificity: It must be capable of being immobilized in an active form to a solid matrix, and the ligand/target complex must be readily dissociable under conditions that release the target molecule in an active form. These criteria are often best fulfilled for protein targets by antibody ligands. Monoclonal antibodies are the ligands of choice for most purposes, especially when a protein of unknown properties is being characterized. Monoclonal antibodies fulfill all the above criteria and also afford the advantage of being available in essentially unlimited supply. Moreover, they are themselves readily purified, often by affinity chromatography. The main disadvantage of monoclonal antibody ligands is that they must possess affinities that are sufficiently high to allow efficient purification and sufficiently low to allow efficient elution. This balance cannot really be tailored, but must be obtained by screening a number of specific monoclonals until empirically one is obtained with the desired properties. Once an appropriate monoclonal antibody has been identified, then in principle the purification of the corresponding antigen is straightforward and is limited in quantity only by the availability of the starting material.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序