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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
- Chromogranin A Monoclonal Antibody, Purified (SIGNET)详细信息:
-
Description 
Purified Monoclonal Antibody against Chromogranin A (LK2H10)
(Formerly Signet Catalog Nos: 215-01, 215-16 L1 Predilute, 215-26 L2 Predilute, 215-36 LV Predilute)Intended Use 
** In Vitro Diagnostic (IVD) ** Clone 
LK2H10 Form 
Purified Antibody (in PBS + 0.1% NaN3 + 1% BSA) Host 
Mouse Species Reactivity 
Chromogranin A Monoclonal Antibody, Purified (SIGNET)Human IsoType 
IgG1/kappa Specificity 
The anti-Chromogranin A antibody recognizes a 68kDa protein tht is expressed by most cells of the diffuse neuroendocrine system. Clone LK2H10 has been shown to stain both normal and neoplastic endocrine tissue containing secretory granules. In tumors with abundant secretory granules, staining is intense and diffuse. In tumors with few granules (e.g. Merkel cell carcinoma), focal staining is observed. Endocrine tissues without secretory granules and non-endocrine tissues do not stain with clone LK2H10.
This antibody was developed using human pheochromocytoma.
Concentrated Format:
SIG-3215-1000 [1 mL]
Prediluted Formats:
SIG-3215-16 [6 mL] is ready to use with Peroxidase Anti- Peroxidase detection systems.
SIG-3215-26 [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultrastreptavidin Detection (SIG-32250).
SIG-3215-36 [6 mL] is ready-to-use with Biotin based detection systems on Ventana ES and Ventana NexES automated immunostainers.Uses 
Chromogranin A Monoclonal Antibody, Purified (SIGNET)This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution 
The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250)
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: Not required
Incubation: 20-60 minutes at room temperature
Notes 
Positive tissue (human): Pancreas - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250)
- Chromogranin A Monoclonal Antibody, Purified (SIGNET)
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