- 询价
- R&D
- 美国/中国
- xy-32614
- 2025年06月25日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- ACUITY Biotin Free Polymer Detection HRP (Cap Gap) Complete 5,000 Tests (SIGNET)详细信息：
-
Description 
ACUITY Biotin Free Polymer Detection HRP System (Cap Gap) Complete, 5,000 Tests
(Formerly Signet Catalog No.: 2614)Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Form Detection Kit Specificity ACUITY Biotin Free Polymer Detection HRP (Cap Gap) Complete 5,000 Tests (SIGNET)Signets ACUITY Detection system is a sensitive biotin-free polymer detection kit that is compatible with antibodies raised in mouse, rat and rabbit. ACUITY contains a horseradish Peroxidase labeled polymer and special blocking solutions to eliminate possible nonspecific background staining.
Kit Contains:- Serum Block, 1000 mL
- Special Block, 1000 mL
- HRP Polymer Link, 1000 mL
- DAB Substrate Buffer, 2x1000 mL
- DAB Chromogen, 100 mL
- Hydrogen Peroxide, 1000 mL
Suggested Working Dilution
ACUITY Biotin Free Polymer Detection HRP (Cap Gap) Complete 5,000 Tests (SIGNET) Protocol:
1. Tissue preparation: Deparaffinize and rehydrate according to the established protocols.
2. Blocking step-1: Incubate with H202 at RT for 15 minutes. Rinse with PBS.
3. Blocking step-2: Apply the Serum Block reagent for 15 minutes.
4. Primary antibody: Apply the primary antibody for 30 minutes.
5. Blocking step-3: Apply the Special Block reagent for 20 minutes. then rinse with PBS (5min/2X).
6. Polymer Link: Apply the Polymer reagent for 30 minutes then rinse with PBS (5min/2X).
7. Chromogen step: Apply DAB for 3-5 minutes then rinse with DI water.
8. Counter stain & mount.Notes Discard unused DAB working solution at the end of the run. Storage Store between 2-8°C. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - ACUITY Biotin Free Polymer Detection HRP (Cap Gap) Complete 5,000 Tests (SIGNET)
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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
assay, frequency analysis of rare cell populations (e.g., antigen-specific responses) which were not possible by bulk assay methods are now possible. Limits of detection are below 1/100,000 rendering the assay uniquely useful for monitoring antigen
,000) prior to visualization with Immobilon Western HRP Chemiluminescent Substrate. The blots were then stripped by (B) low pH or (C) heat/detergent and re-exposed to the secondary antibody and the Immobilon Western substrate to demonsrate complete
BioID: A Screen for Protein‐Protein Interactions
for each experimental condition (cells expressing BioID constructs or control cells) Complete medium 1 mM (20×) biotin (see recipe
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