• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography

        互联网

        2239
        In the past, isolation of a pure protein demanded many hours to develop a purification protocol, which included one or more cycles of time-consuming chromatography, only to have a final product that was enriched rather than purified. The development of a purification procedure had to be repeated for each protein of interest. Almost as much time was spent on purification as characterization of the protein and reproducibility varied from batch to batch of starting material. After techniques to produce monoclonal antibodies (MAbs) were developed, purification of antigens has been facilitated with MAbs coupled to Sepharose 4B (Pharmacia Biotech Inc., Piscataway, NJ). MAb reagents coupled to this support matrix require reduced time to purify proteins. Taking advantage of the exquisite specificity afforded by each antibody results in a final purified product that binds specifically to that MAb. Using immunoaffinity chromatography, a single antigen can be separated from very complex mixtures (e.g., detergent-disrupted cell lysates made from tissue-culture cells), and whole blood and organs (e.g., thymus and spleen).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序