Stemolecule™ IDE-2

Stemolecule™ IDE-2详细信息：
Product Overview

Stemolecule™ IDE-2 was identified in a high throughput screen as an inducer of definitive endoderm (IDE) from embryonic stem (ES) cells1. IDE-2 is able to generate Sox17+/FoxA2+ cells to an equal or greater efficiency than that of protein (i.e. Activin A or Nodal) inducers of definitive endoderm in mouse or human ES cell cultures1. It has an EC50 of 223 nM and functions in part via activation of TGF-β signaling, as evident by Smad2 phosphorylation1. Endoderm chemically induced by IDE-2 can continue to differentiate into pancreatic progenitors in vitro and contribute to gut tube formation in vivo when injected into the developing gut tube of mouse embryos1.

Stemolecule™ IDE-2Product Specifications

Size / Quantity 2 mg
Alternate Name 7-(2-cyclopentylidenehydrazinyl)-7-oxoheptanoic acid, Induce definitive endoderm-2
CAS Number N/A
Chemical Formula C12H20N2O3
Structure [structure graphics]
Formulation White Powder
Stemolecule™ IDE-2Molecular Weight 240.30
Purity >99%
Solubility Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 832 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage Store at 4°C. Protect from light. Following reconstitution, store aliquots at -20°C.
Stability Stock solutions stable at -20°C for 6 months.
Quality Control The purity of IDE-2 was determined by HPLC analysis. The accurate mass and structure of IDE-2 were determined by mass spectrometry and NMR, respectively. Cellular toxicity of IDE-2 was tested on mouse ES cells.
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