PI(4,5)P2 Mass ELISA

Description: The PI(4,5)P₂ ELISA measures PI(4,5)P₂ from cell extractions. The assay is a competitive ELISA where the signal is inversely proportional to the amount of PI(4,5)P₂ measured.

This allows the user to measure the amount of PI(4,5)P₂ extracted from cells by means of standard 96 well ELISA format, eliminating the need for radioactivity and thin layer chromatography. Once PI(4,5)P₂ has been extracted from cellular samples, it is incubated with a PI(4,5)P₂ detector protein, then added to the PI(4,5)P₂-coated plate for competitive binding. A peroxidase-linked secondary detection reagent and colorimetric substrate is used to detect PI(4,5)P₂ detector protein binding to the plate.The colorimetric signal is inversely proportional to the amount of PI(4,5)P₂ extracted from cells.

Reference

• Nonradioactive Quantification of Phosphoinositide 3-kinase (PI3K) Products from Breast Cancer Cell Lines andQuantification of PI3-Kinase Products from Breast Cancer Cell Lines

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