Stemolecule™ Doxycycline hycla

Stemolecule™ Doxycycline hyclate详细信息：
Product Overview

Stemolecule™ Doxycycline hyclate (Dox) is a broad spectrum antibiotic derivative of tetracycline and an inhibitor of matrix metalloproteinases in vivo. Tetracycline-controlled transcriptional activation is a method of inducible expression whereby transcription is reversibly turned on or off in the presence of tetracycline or one of its derivatives such as Dox1. Dox-inducible lentiviral reagents such as the Stemgent® iPSC Generation Dox Inducible Lentivirus products are used to induce the expression of virally transduced genes and generate induced pluripotent stem (iPS) cells from somatic cells (in a process referred to as reprogramming2,3) by adding Dox to the cell culture medium4-9. Stemolecule™ Doxycycline hyclate is the recommended inducer for all of the Stemgent® iPSC Generation Dox Inducible products. In the iPSC generation protocol, addition of Dox to the culture medium of transduced cells induces the reverse tetracycline transactivator (rtTA) to bind to the Tet response element and results in the transcriptional activation for each of the factors implicated in the reprogramming process. Conversely, Dox can be removed from the medium to suppress transcriptional activation of the reprogramming factors.

Stemolecule™ Doxycycline hyclateProduct Specifications

Size 10 mg
Lot Number 1203
Quantity / Size 10 mg
Alternate Names (4S,4aR,5S,5aR,6R,12aS)-4-(dimethylamino)-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-1,4,4a,5,5a,6,11,12a-octahydrotetracene-2-carboxamide; Doxycycline hydrochloride hemiethanolate hemihydrate; Dox
Chemical Formula C22H24N2O8 • HCl • ½(H2O) • ½(C2H6O)
Structure
Stemolecule™ Doxycycline hyclateMolecular Weight 512.94
CAS Number 24390-14-5
Purity ≥98% (TLC)
Formulation Yellow to yellow with a green cast powder
Solubility Soluble in water
*DMSO upper limit tolerance is <0.5% in most cells.
Storage Store at 4°C. Protect from light. Following reconstitution, store aliquots at -20°C.
Stability Stock solutions stable at -20°C for 6 months.
Quality control The purity of Stemolecule™ Doxycycline hyclate was determined by TLC analysis. The accurate mass and structure were determined by mass spectrometry and NMR, respectively. Cellular toxicity was tested on mouse embryonic stem cells.
Stemolecule™ Doxycycline hyclate
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