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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
PI(3,4)P2 Mass ELISA
- 库存：
1
- 供应商：
上海信裕
- PI(3,4)P2 Mass ELISA详细信息：
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Description: The PI(3,4)P2 Mass ELISA measures the amount of PI(3,4)P2 extracted from cells by means of a competitive ELISA, (96 well format).
The assay is a competitive ELISA where the signal is inversely proportional to the amount of PI(3,4)P2produced. This allows the user to measure the amount of PI(3,4)P2 extracted from cells by means of standard 96 well ELISA format, eliminating the need for radioactivity and thin layer chromatography. Once PI(3,4)P2 has been extracted from cellular samples, it is incubated with a PI(3,4)P2 detector, then added to the PI(3,4)P2-coated plate for competitive binding. A peroxidase-linked secondary detection reagent and colorimetric substrate is used to detect PI(3,4)P2 detector binding to the plate. The colorimetric signal is inversely proportional to the amount of PI(3,4)P2 extracted from cells.
Reference
- Nonradioactive Quantification of Phosphoinositide 3-kinase (PI3K) Products from Breast Cancer Cell Lines andQuantification of PI3-Kinase Products from Breast Cancer Cell Lines
- PI(3,4)P2 Mass ELISA
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文献和实验Mass Measurement of Key Phosphoinositide Cycle Intermediates
activation of membrane-associated enzymic activities act on the minor membrane phospholipid phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2 ] to generate three second messenger molecules: inositol 1,4,5-trisphosphate [Ins(1,4,5)P3
of each component. 2D gel electrophoresis is generally used as a component of proteomics and is the step used for the isolation of proteins for further characterisation by mass spectroscopy. In the lab this technique is used for 2 main purposes, firstly
of each component.2D gel electrophoresis is generally used as a component of proteomics and is the step used for the isolation of proteins for further characterisation by mass spectroscopy. In the lab this technique is used for 2 main purposes, firstly for the large
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