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- V900891
- 2026年01月03日
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- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
1年
- 英文名：
Collagenase I
- 库存：
100⁺瓶
- 供应商：
上海睿安生物13611631389
- 规格：
1g/瓶
Sigma货号V900891胶原酶I型(Collagenase I)上海睿安生物13611631389
说明
应用:来自溶组织梭菌(Clostridium histolyticum)的胶原酶，可用于从许多类型的动物组织中分离细胞。VETECI型胶原酶具有胶原酶、酪蛋白酶、梭菌蛋白酶和胰蛋白酶活性的原始平衡，建议用于上皮细胞、肝细胞、肺细胞和肾上腺原代细胞的分离。
生化/生理作用:每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双(β-氨基乙基醚)-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯(qiú)基乙酸和8-羟基喹(kuí)啉抑制。
质量:也具有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性。
单位定义:一个胶原蛋白消化单元(CDU)从牛跟腱胶原蛋白中释放的肽链量和1.0μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0μmole酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0μmole BAEE。
同行评审论文
G Protein-Coupled Estrogen Receptor 30 Reduces Transverse Aortic Constriction-Induced Myocardial Fibrosis in Aged Female Mice by Inhibiting the ERK1/2 -MMP-9 Signaling Pathway.
Xiaowu Wang et al.
Frontiers in pharmacology, 12, 731609-731609 (2021-11-23)
The incidence of cardiovascular diseases was significantly increased in postmenopausal women. The protection of estrogen in the cardiovascular system has been further reported for decades. Although menopausal hormone therapy has been used in many clinical trials, the debatable results indicate
The long noncoding RNA MyHC IIA/X-AS contributes to skeletal muscle myogenesis and maintains the fast fiber phenotype.
Mingle Dou et al.
The Journal of biological chemistry, 295(15), 4937-4949 (2020-03-11)
Mammalian skeletal muscles comprise different types of muscle fibers, and this muscle fiber heterogeneity is generally characterized by the expression of myosin heavy chain (MyHC) isoforms. A switch in MyHC expression leads to muscle fiber-type transition under various physiological and
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Haijian Cai et al.
Journal of cardiovascular pharmacology, 80(1), 95-109 (2022-05-06)
Dihydroartemisinin (DHA) is an active form of artemisinin extracted from the traditional Chinese medicine Artemisia annua , which is used to treat malaria. Previous studies have shown that DHA has a therapeutic effect on pulmonary hypertension (PH), but its specific
Stromal PD-1+ tumor-associated macrophages predict poor prognosis in lung adenocarcinoma.
Lu Chen et al.
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Immunotherapies targeting programmed cell death protein 1 (PD-1)/PD-1 ligand (PD-L1) axis have been emerging as a promising therapeutic strategy to treat lung cancer. PD-1 is preferentially expressed by activated T lymphocytes; but whether/how its expression by tumor-associated macrophages (TAMs) in
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Mitochondrial unfolding protein response (UPRmt) effectively resists the pathological cardiac hypertrophy and improves the mitochondrial function. However, the specific activation mechanism and drugs that can effectively activate UPRmt in the cardiac muscle are yet to be elucidated. The aim of
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文献和实验该产品被引用文献
Sigma货号V900891胶原酶I型(Collagenase I)上海睿安生物13611631389
说明
应用:来自溶组织梭菌(Clostridium histolyticum)的胶原酶，可用于从许多类型的动物组织中分离细胞。VETECI型胶原酶具有胶原酶、酪蛋白酶、梭菌蛋白酶和胰蛋白酶活性的原始平衡，建议用于上皮细胞、肝细胞、肺细胞和肾上腺原代细胞的分离。
生化/生理作用:每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双(β-氨基乙基醚)-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯(qiú)基乙酸和8-羟基喹(kuí)啉抑制。
质量:也具有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性。
单位定义:一个胶原蛋白消化单元(CDU)从牛跟腱胶原蛋白中释放的肽链量和1.0μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0μmole酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0μmole BAEE。
同行评审论文
G Protein-Coupled Estrogen Receptor 30 Reduces Transverse Aortic Constriction-Induced Myocardial Fibrosis in Aged Female Mice by Inhibiting the ERK1/2 -MMP-9 Signaling Pathway.
Xiaowu Wang et al.
Frontiers in pharmacology, 12, 731609-731609 (2021-11-23)
The incidence of cardiovascular diseases was significantly increased in postmenopausal women. The protection of estrogen in the cardiovascular system has been further reported for decades. Although menopausal hormone therapy has been used in many clinical trials, the debatable results indicate
The long noncoding RNA MyHC IIA/X-AS contributes to skeletal muscle myogenesis and maintains the fast fiber phenotype.
Mingle Dou et al.
The Journal of biological chemistry, 295(15), 4937-4949 (2020-03-11)
Mammalian skeletal muscles comprise different types of muscle fibers, and this muscle fiber heterogeneity is generally characterized by the expression of myosin heavy chain (MyHC) isoforms. A switch in MyHC expression leads to muscle fiber-type transition under various physiological and
Dihydroartemisinin Attenuates Hypoxia-Induced Pulmonary Hypertension Through the ELAVL2/miR-503/PI3K/AKT Axis.
Haijian Cai et al.
Journal of cardiovascular pharmacology, 80(1), 95-109 (2022-05-06)
Dihydroartemisinin (DHA) is an active form of artemisinin extracted from the traditional Chinese medicine Artemisia annua , which is used to treat malaria. Previous studies have shown that DHA has a therapeutic effect on pulmonary hypertension (PH), but its specific
Stromal PD-1+ tumor-associated macrophages predict poor prognosis in lung adenocarcinoma.
Lu Chen et al.
Human pathology, 97, 68-79 (2020-01-12)
Immunotherapies targeting programmed cell death protein 1 (PD-1)/PD-1 ligand (PD-L1) axis have been emerging as a promising therapeutic strategy to treat lung cancer. PD-1 is preferentially expressed by activated T lymphocytes; but whether/how its expression by tumor-associated macrophages (TAMs) in
Novel PGC-1α/ATF5 Axis Partly Activates UPRmt and Mediates Cardioprotective Role of Tetrahydrocurcumin in Pathological Cardiac Hypertrophy.
Bing Zhang et al.
Oxidative medicine and cellular longevity, 2020, 9187065-9187065 (2021-01-12)
Mitochondrial unfolding protein response (UPRmt) effectively resists the pathological cardiac hypertrophy and improves the mitochondrial function. However, the specific activation mechanism and drugs that can effectively activate UPRmt in the cardiac muscle are yet to be elucidated. The aim of
相关实验
基 解离培养基 OCO培养基 细胞冻存液 近岸蛋白产品货号 基础培养基 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 penicillin-streptomycin 2% - 1% - FBS 10% - - 30% Collagenase IV - 1mg/ml - - L-glutamine - - 1×
, 胶原酶(Collagenase type Ⅱ) , 海胺(BenzethoniumHydroxide) 均为SIGMA 公司产品; 牛血清白蛋白(Albumin Bovine) 为Amresco 公司产品, D - [ U - 14C] 葡萄糖[ 1mmol/ L含U - 14 C 9. 176 GBq (即248mCi) ] 购自中国医学科学院放射医学研究所。Beck2manLsl801 液体闪烁谱仪与倒置生物显微镜XSZ - D2。实验动物与饲料加工: 雄性SD大鼠(体重160g 左右
Sigma分装 B1060 CHAPS 1g/2.5g/10g 180/300/1000 Amresco分装 B1063 Collagenase V （ V 型胶原酶 ） 100mg 500 Sigma分装 B1065
文献支持
Sigma货号V900891-1g胶原酶I型(Collagenase I)上海睿安生物13611631389
￥2684.21
