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- 2025年10月24日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-23103R |
| 英文名称 | Ki-67 Rabbit pAb |
| 中文名称 | Ki67抗体 |
| 英文别名 | Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_MOUSE. |
| 产品应用 | ICC/IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Ki-67 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 358 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Cell division Cancer > Tumor biomarkers Cell Biology > Cell Cycle > Markers Neuroscience > Cell Type Marker > Neuron marker > Soma marker Neuroscience > Neurology process > Neurogenesis Tags & Cell Markers > Cell Type Markers > Replication |
| 亚细胞定位 | Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes. |
| 相似性 | Contains 1 FHA domain. |
| 功能 | Thought to be required for maintaining cell proliferation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
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文献和实验[IF={{ 9.4 }}] {Xia Lei. et al.pH-responsive photothermal effect and heterojunction formation for tumor-specific pyroelectrodynamic and nanozyme-catalyzed starvation therapy..Acta Biomaterialia.2025 Mar 18:S1742-7061(25)00204-1.} {IHC,IF} {Mouse}
[IF={{ 7.242 }}] {Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 Mar;:} {IF} {Mouse}
[IF={{ 6.819 }}] {Hongchao Xu. et al. A facile method for anti-cancer drug encapsulation into polymersomes with a core-satellite structure. DRUG DELIV. 2022 Jul 29} {IHC} {Mouse}
[IF={{ 6.656 }}] {Ning Han. et al. Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy. PHYTOMEDICINE. 2023 Jan;:154682} {WB} {Mouse}
[IF={{ 6.6 }}] {Guo-Bin Ding. et al. Biosynthesized tumor acidity and MMP dual-responsive plant toxin gelonin for robust cancer therapy. BIOMATER SCI-UK. 2023 Dec;:} {IHC} {Mouse}
-30% 的乳腺癌存在 HER2 基因扩增，从而使细胞膜上 HER2 蛋白的显著过表达。这引发强烈的、持续的下游促增殖信号，导致侵袭性更强的肿瘤表型[10]。此外，HER2 的过表达或突变与胃癌、胃食管癌、肺癌、子宫内膜癌及卵巢癌也密切相关[11]。 图 6. 人乳腺癌组织的 HER2 免疫组化分析 (HY-P80658)。 Ki-67 Ki-67——细胞增殖相关的标志性蛋白。 定位：细胞核 功能：Ki-67 是重要的细胞
T-cell dependent antigen specific in situ staining (Pe)
Double staining for Ag and Ki-67, TUNEL or BCL-6. Cut frozen tissue sections (spleen, lymph nodes) in a cryostat at ~ 4µm, on plain glass slides (Adhesive -coated slides are sometimes too hydrophobic, but fine
-mouse-mab.html 可用于乳腺癌组织的免疫组化分析 Ki67 Antidiv (YA322) (HY-P80506) www.medchemexpress.cn/antibody/ki67-rabbit-mab.html 可用于乳腺癌组织的免疫组化分析 p53 Antidiv (YA250) (HY-P80257) www.medchemexpress.cn/antibody/p53-rabbit-mab.html 可用于乳腺癌组织的免疫组化分析 Cytokeratin 7 Antidiv
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