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MMP2 Rabbit pAb(bs-4599R)-50ul

/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-4599R
更新日期2026年10月17日

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规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-4599R
英文名称	MMP-2 Rabbit pAb
中文名称	基质金属蛋白酶2抗体
英文别名	MMP2; MMP 2; 72 kDa gelatinase; 72kD type IV collagenase; CLG 4; CLG 4A; CLG4; CLG4A; Collagenase Type 4 alpha; Collagenase type IV A; Gelatinase A; Gelatinase alpha; Gelatinase neutrophil; Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase; Matrix metalloproteinase 2(gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); Matrix Metalloproteinase 2; Matrix metalloproteinase II; MMP 2; MMP II; MONA; Neutrophil gelatinase; TBE 1.
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Human (Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep)
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human MMP-2
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	62 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cancer > Cancer Metabolism > Response to hypoxia Cancer > Invasion/microenvironment > ECM > Extracellular matrix > MMPs Cancer > Tumor biomarkers > Enzymes > MMPs Cardiovascular > Angiogenesis > Adhesion / ECM > Matrix Metalloproteinases > MMP Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Metalloprotease > MMPs Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Metabolism > Types of disease > Cancer
亚基	Interacts (via the C-terminal hemopexin-like domains-containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B.
亚细胞定位	Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes._x000D_ Isoform 2: Cytoplasm. Mitochondrion.
组织特异性	Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
翻译后修饰	Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro._x000D_ The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
相似性	Belongs to the peptidase M10A family. _x000D_ Contains 3 fibronectin type-II domains. _x000D_ Contains 4 hemopexin-like domains.
功能	Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-\|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro._x000D_ PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels._x000D_ Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

应用	推荐稀释比例
{WB}	{1:500-2000}
{IHC-P}	{1:100-500}
{IHC-F}	{1:100-500}
{ICC/IF}	{1:100-500}
{IF}	{1:100-500}
{Flow-Cyt}	{1μg/Test}

Sample:
_x000D_ A431(Human) Cell Lysate at 30 ug
_x000D_ Mcf-7(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-MMP-2 (bs-4599R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 62 kD
_x000D_ Observed band size: 62 kD

Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
_x000D_ Primary Antibody (green line): Rabbit Anti- MMP2 antibody (bs-4599R),Dilution: 1μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
_x000D_

Tissue/cell: U-87MG cell; 4% P‌‌araformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (MMP2)polyclonal Antibody, Unconjugated (bs-4599R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Sample:
_x000D_ SY5Y (Human) Cell Lysate at 30 ug
_x000D_ U2os (Human) Cell Lysate at 30 ug
_x000D_ U251 (Human) Cell Lysate at 30 ug
_x000D_ Siha (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti- MMP-2 (bs-4599R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 62 kD
_x000D_ Observed band size: 60 kD
_x000D_

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