- 询价
- BIO-RAD
- 1708841
- 2025年11月15日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
Script反转录试剂盒易于使用，重复性高，不管目标基因的丰度如何、起始的RNA输入量如 何，都可保证反转录效率的一致性，确保cDNA的差异能真实反应RNA表达的差异。
iScript试剂盒的特点包含
■ 反转录酶具有RNase H＋活性，确保1:1的RNA:cDNA转化比例，以得到更高灵敏度和更准确的结果
■ 优化的oligo(dT)和随机引物混合的引物策略可以保证完整和无偏差覆盖反转录区域
■ 添加RNA酶抑制剂可以在反应液配置和cDNA生成过程中有效防止RNA降解
Choose iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR. This single-tube supermix contains all the components for reverse transcription except RNA template.
Key Features and Benefits
- Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
- Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 µg–1 pg total input RNA
- Amplify cDNA for genes with low expression levels
- Fast 26-minute 1-tube cDNA synthesis protocol
- 50 reactions of no-RT controls for optional detection of gDNA amplicon contamination
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Clonality - X Chromosome Inactivation Assay
) Forward and reverse primers, each 20 µM (Human Androgen Receptor Gene (HUMARA) CAG trinucleotide repeat microsatellite primers are described in Allen et al) AmpliTaq Gold, 5 U/ml (Perkin Elmer) a 32P-dCTP, 20 µCi/µl (NEN) Gel-Mix 6 (Life Technologies
Reverse Transcription of RNA- 20l rxn
0.01l is the 1st strand derived from 0.00075 ug of Poly A+ RNA which is the equivalent of 0.15ug of total RNA (X-H''s method works out to use the eqivalent of 0.12ug of total RNA in the RT-PCR) Controls should include: 1) primers alone
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
1.5 ml 1.2 mM EDTA 0.5M stock 120 µl 1.1% Triton X-100 10% stock 5.5 ml 0.01% SDS 10% stock 50 µl
技术资料暂无技术资料 索取技术资料
