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- BIO-RAD
- 1725035
- 2025年11月26日
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- 详细信息
- 文献和实验
- 技术资料
500 x 20 µl reactions, 5 x 400 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 5 x 400 µl no-RT control supermix; 5 x 50 µl DNase; 5 x 150 µl DNase buffer
描述
Use iScript gDNA Clear cDNA Synthesis Kit for easy and rapid generation of qPCR-ready cDNA free of genomic DNA (gDNA) for accurate gene expression analysis by real-time PCR.
Features and Benefits
- Effective gDNA removal in purified RNA samples
- Proprietary DNase buffer for maintaining RNA integrity during gDNA removal
- Convenient all-in-one, single-tube RT supermix
- Ready-to-use no-RT control supermix for monitoring gDNA contamination
- gDNA-free cDNA ready in 36 min
gDNA-Free cDNA Ready in 36 Minutes
This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.
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文献和实验of mRNA) or random hexamers; the kit used here contains both kinds); nucleotides for DNA synthesis (dNTPs); MgC2 and buffers required by the enzyme, which are all present in the 5x Reaction Mix. Materials * iScript cDNA Synthesis Kit (BIORAD
cDNA Synthesis from MOLT-4 Cells
First strand of cDNA synthsis 1.Add 2 μl of Not I primer-adapter to a sterile 1.5 ml RNAase free tube, add 4.5 μl mRNA of molt4 cells (~5.2 ug), add 0.5 μl DEPC-H2O. 2. Heat the mixture at 70℃ for 10 min, and quick chill on ice, collect
from the handbook and annotated based on experience with the kit. Protocol: QIAprep Spin Miniprep Kit Using a Microcentrifuge This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1�5 ml overnight cultures of E. coli in LB
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