重组新冠病毒Coronavirus spike（XBB.1.5）蛋白

重组新冠病毒Coronavirus spike蛋白（产品说明）
蛋白名称：SARS-COV-2 XBB.1.5 (Omicron) Spike S1+S2 trimer Protein (His & AVI Tag), Biotinylated (MALS-verified)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (XBB.1.5) Spike S1+S2 trimer (YP_009724390.1, with mutations T19I, del24-26, A27S, V83A, G142D, Y144del, H146Q, Q183E, V213E, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, R682G, R683S, R685S, N764K, D796Y, F817P, A892P, A899P, A942P, Q954H, K986P, V987P, N969K and furin cleavage site mutants) was expressed with bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The mutations were identified in the SARS-CoV-2 variant (known as variant XBB.1.5).The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 XBB.1.5 Spike S1+S2 trimer (His & AVI Tag), Biotinylated (Cat: 40589-V49H18-B), the EC50 is 35-100 ng/mL (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Val 16
蛋白分子量：The recombinant SARS-CoV-2 (XBB.1.5) Spike S1+S2 trimer consists of 1242 amino acids and predicts a molecular mass of 138.09 kDa. The molecular weight of this protein is around 530.7 kDa verified by SEC-MALS.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Met1-Gln1208