- ￥5820 - 38730
- Sino Biological
- 北京
- 40589-V08H4
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant SARS-CoV-2 Spike S1+S2 ECD mutants-His Recombinant Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/100.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥38730.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥5820.0 |
SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein: 产品信息
纯度> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Measured by its binding ability in a functional ELISA.
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (40589-V08H4), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (40589-V08H4) is 10-50 ng/mL.
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD) (YP_009724390.1) (Met1-Pro1213(R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)) was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
NP No.YP_009724390.1
表达宿主HEK293 Cells
种属2019-nCoV
预测 N 端Val 16
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD(R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P), His tag) consists of 1248 amino acids and predicts a molecular mass of 138.5 kDa.
缓冲液Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
纯度> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Measured by its binding ability in a functional ELISA.
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (40589-V08H4), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (40589-V08H4) is 10-50 ng/mL.
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD) (YP_009724390.1) (Met1-Pro1213(R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)) was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
NP No.YP_009724390.1
表达宿主HEK293 Cells
种属2019-nCoV
预测 N 端Val 16
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD(R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P), His tag) consists of 1248 amino acids and predicts a molecular mass of 138.5 kDa.
缓冲液Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验该产品被引用文献
1, Huang, J; et al. Simultaneous enrichment and separation of neutral and sialyl glycopeptides of SARS-CoV-2 spike protein enabled by dual-functionalized Ti-IMAC material. Analytical and bioanalytical chemistry, PMID: 34155551
2, Chang, X; et al. A Novel Double Mosaic Virus-like Particle-Based Vaccine against SARS-CoV-2 Incorporates Both Receptor Binding Motif (RBM) and Fusion Domain. Vaccines, PMID: 34835218
3, Abdillah, DA; et al. Spike protein of SARS-CoV-2 suppresses gonadotrophin secretion from bovine anterior pituitaries. The Journal of reproduction and development, PMID: 35082199
4, Barber, KW; et al. CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling. Cell reports methods, PMID: 36313802
5, Park, KS; et al. Ultra-sensitive label-free SERS biosensor with high-throughput screened DNA aptamer for universal detection of SARS-CoV-2 variants from clinical samples. Biosensors & bioelectronics, PMID: 36940632
6, Adams, LE; et al. Fc-mediated pan-sarbecovirus protection after alphavirus vector vaccination. Cell reports, PMID: 37000623
7, Tong, X; et al. Waning and boosting of antibody Fc-effector functions upon SARS-CoV-2 vaccination. Nature communications, PMID: 37443074
8, Ou, BS; et al. Saponin Nanoparticle Adjuvants Incorporating Toll-Like Receptor Agonists Improve Vaccine Immunomodulation. bioRxiv : the preprint server for biology, PMID: 37577608
9, Kehrer, T; et al. Impact of SARS-CoV-2 ORF6 and its variant polymorphisms on host responses and viral pathogenesis. Cell host & microbe, PMID: 37738983
10, Aydillo, T; et al. Concomitant administration of seasonal influenza and COVID-19 mRNA vaccines. Emerging microbes & infections, PMID: 38054302
11, Mayer, DP; et al. A novel microporous biomaterial vaccine platform for long-lasting antibody mediated immunity against viral infection. Journal of controlled release : official journal of the Controlled Release Society, PMID: 38734312 1
2, Meany, EL; et al. Generation of an inflammatory niche in an injectable hydrogel depot through recruitment of key immune cells improves efficacy of mRNA vaccines. bioRxiv : the preprint server for biology, PMID: 39026835 1
3, Guenthoer, J; et al. The S2 subunit of spike encodes diverse targets for functional antibody responses to SARS-CoV-2. PLoS pathogens, PMID: 39093891 1
4, Ou, BS; et al. Saponin nanoparticle adjuvants incorporating Toll-like receptor agonists drive distinct immune signatures and potent vaccine responses. Science advances, PMID: 39110802 1
5, Jung, W; et al. SARS-CoV-2 infection prior to vaccination amplifies Fc-mediated humoral profiles in an age-dependent manner. Cell reports, PMID: 39213155 1
6, Ramírez, MA; et al. Co-administration of recombinant BCG and SARS-CoV-2 proteins leads to robust antiviral immunity. Vaccine, PMID: 39178767 1
7, Tong, X; et al. Compartment-specific antibody correlates of protection to SARS-CoV-2 Omicron in macaques. iScience, PMID: 39224511 1
8, Beavis, AC; et al. A parainfluenza virus 5 (PIV5)-vectored intranasal SARS-CoV-2 vaccine (CVXGA1) elicits protective and long-lasting immunity in nonhuman primates. Journal of virology, PMID: 40116506 1
9, Meany, EL; et al. Generation of an inflammatory niche in a hydrogel depot through recruitment of key immune cells improves efficacy of mRNA vaccines. Science advances, PMID: 40215318
20, Félix, P; et al. Exploring TLR agonists as adjuvants for COVID-19 oral vaccines. Vaccine, PMID: 40184639
21, Thompson, A; et al. Identification of an Unnatural Sulfated Monosaccharide as a High-Affinity Ligand for Pan-Variant Targeting of SARS-CoV-2 Spike Glycoprotein. ACS chemical biology, PMID: 40358361 2
2, Mak, WA; et al. Seasonal Coronavirus-Induced Immunological Imprinting and Previous Herpesvirus Infections in Patients With Long COVID. Journal of medical virology, PMID: 40884088
2, Chang, X; et al. A Novel Double Mosaic Virus-like Particle-Based Vaccine against SARS-CoV-2 Incorporates Both Receptor Binding Motif (RBM) and Fusion Domain. Vaccines, PMID: 34835218
3, Abdillah, DA; et al. Spike protein of SARS-CoV-2 suppresses gonadotrophin secretion from bovine anterior pituitaries. The Journal of reproduction and development, PMID: 35082199
4, Barber, KW; et al. CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling. Cell reports methods, PMID: 36313802
5, Park, KS; et al. Ultra-sensitive label-free SERS biosensor with high-throughput screened DNA aptamer for universal detection of SARS-CoV-2 variants from clinical samples. Biosensors & bioelectronics, PMID: 36940632
6, Adams, LE; et al. Fc-mediated pan-sarbecovirus protection after alphavirus vector vaccination. Cell reports, PMID: 37000623
7, Tong, X; et al. Waning and boosting of antibody Fc-effector functions upon SARS-CoV-2 vaccination. Nature communications, PMID: 37443074
8, Ou, BS; et al. Saponin Nanoparticle Adjuvants Incorporating Toll-Like Receptor Agonists Improve Vaccine Immunomodulation. bioRxiv : the preprint server for biology, PMID: 37577608
9, Kehrer, T; et al. Impact of SARS-CoV-2 ORF6 and its variant polymorphisms on host responses and viral pathogenesis. Cell host & microbe, PMID: 37738983
10, Aydillo, T; et al. Concomitant administration of seasonal influenza and COVID-19 mRNA vaccines. Emerging microbes & infections, PMID: 38054302
11, Mayer, DP; et al. A novel microporous biomaterial vaccine platform for long-lasting antibody mediated immunity against viral infection. Journal of controlled release : official journal of the Controlled Release Society, PMID: 38734312 1
2, Meany, EL; et al. Generation of an inflammatory niche in an injectable hydrogel depot through recruitment of key immune cells improves efficacy of mRNA vaccines. bioRxiv : the preprint server for biology, PMID: 39026835 1
3, Guenthoer, J; et al. The S2 subunit of spike encodes diverse targets for functional antibody responses to SARS-CoV-2. PLoS pathogens, PMID: 39093891 1
4, Ou, BS; et al. Saponin nanoparticle adjuvants incorporating Toll-like receptor agonists drive distinct immune signatures and potent vaccine responses. Science advances, PMID: 39110802 1
5, Jung, W; et al. SARS-CoV-2 infection prior to vaccination amplifies Fc-mediated humoral profiles in an age-dependent manner. Cell reports, PMID: 39213155 1
6, Ramírez, MA; et al. Co-administration of recombinant BCG and SARS-CoV-2 proteins leads to robust antiviral immunity. Vaccine, PMID: 39178767 1
7, Tong, X; et al. Compartment-specific antibody correlates of protection to SARS-CoV-2 Omicron in macaques. iScience, PMID: 39224511 1
8, Beavis, AC; et al. A parainfluenza virus 5 (PIV5)-vectored intranasal SARS-CoV-2 vaccine (CVXGA1) elicits protective and long-lasting immunity in nonhuman primates. Journal of virology, PMID: 40116506 1
9, Meany, EL; et al. Generation of an inflammatory niche in a hydrogel depot through recruitment of key immune cells improves efficacy of mRNA vaccines. Science advances, PMID: 40215318
20, Félix, P; et al. Exploring TLR agonists as adjuvants for COVID-19 oral vaccines. Vaccine, PMID: 40184639
21, Thompson, A; et al. Identification of an Unnatural Sulfated Monosaccharide as a High-Affinity Ligand for Pan-Variant Targeting of SARS-CoV-2 Spike Glycoprotein. ACS chemical biology, PMID: 40358361 2
2, Mak, WA; et al. Seasonal Coronavirus-Induced Immunological Imprinting and Previous Herpesvirus Infections in Patients With Long COVID. Journal of medical virology, PMID: 40884088
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Sino Biological 新冠病毒Spike重组蛋白 高纯度突变体 新冠S蛋白
￥5820 - 38730
